Pengaruh Durasi Perendaman Oksidasi Madu terhadap Perubahan Warna Gigi in Vitro

Erma Sofiani, Gurnita Swasti Yudasmara

Abstract


Madu merupakan salah satu bahan alternatif yang aman dalam pemutihan gigi. Penelitian ini bertujuan untuk mengetahui pengaruh lama perendaman hasil oksidasi madu terhadap perubahan warna enamel gigi. Jenis penelitian ini adalah eksperimental laboratorium secara in vitro. Sampel gigi dibagi 5 kelompok masingmasing 5 buah gigi yaitu, kelompok 1 (kontrol negatif): sampel direndam dalam aquadest steril, kelompok 2 (kontrol positif): sampel direndam dalam hidrogen peroksida 3%, kelompok 3: sampel direndam larutan madu selama 30 menit, kelompok 4: sampel direndam  larutan madu selama 45 menit dan kelompok 5: sampel direndam larutan madu selama 60 menit. Sebelum dilakukan perendaman, larutan madu dilakukan sentrifugasi selama 30 menit dengan kecepatan 500 rpm. Pengukuran derajat perubahan warna gigi menggunakan shade guide dan spectrophotometer. Analisis data menggunakan uji Wilcoxon, KruskallWallis, dan Mann-Whitney U. Hasil penelitian menunjukkan bahwa terdapat perubahan warna antara sebelum dan sesudah perendaman pada kelompok 60 menit berdasarkan uji analisis Wilcoxon. Terdapat pengaruh lama perendaman terhadap perubahan warna pada pengukuran menggunakan shade guide berdasarkan uji analisis Kruskal-Wallis, namun tidak terdapat pengaruh lama perendaman pada pengukuran menggunakan spectrophotometer. Disimpulkan bahwa terdapat perubahan warna gigi setelah 2 minggu perendaman menggunakan hasil oksidasi madu kelengkeng pada kelompok 60 menit.

One of the alternative safety materials can be used for bleaching is honey. The aims of this study is determine the effect duration of immersion with honey oxidation toward discoloration of tooth. This research is a experimental research laboratory in vitro. Sample divided into 5 groups, consisting 5 pieces of tooth. Group 1: samples were soaked in sterile distilled water, group 2: soaked with H2O2 3%, group 3-5: soaked in the solution of honey oxidation  for 30 minutes, 45 minutes and 60 minutes. Before soaked, honey were centrifuged  for 30 minutes with speed of 500 rpm. Measurement of degree of color change using shade guide and  spectrophotometer. Data analyze using Wilcoxon test, Kruskall-Wallis, and Mann-Whitney U. Results showed there is discoloration between before and after soaked in 60 minute group using Wilcoxon test, there is effect duration of soaking toward color changes using shade guide, but there is no effect using spectrophotometer based on Kruskall Wallis test. It was concluded that there was discoloration of the tooth after 2 weeks of immersion using the result of oxidation of kelengkeng’s honey in group 60 minutes.

Keywords


Duration of immersion; bleaching; hydrogen peroxide; honeyKata kunci: Lama perendaman; bleaching; hidrogen peroksida; madu

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