Antioxidant and Chemoprevention Activity of Camelia Sinensis-Annona muricata Extract Combination against WiDr Cells Line
DOI:
https://doi.org/10.18196/mmjkk.v21i2.11158Keywords:
Annona muricata L, antioxidants, cytotoxic, Camelia Sinensis L, WiDr cellAbstract
Antioxidant compounds have an essential role in inhibiting the process of cell proliferation and have a chemopreventive effect. This study aims to trace the presence of antioxidant compounds allegedly contained in tea leaves (Camelia Sinensis L.) and soursop leaves (Anonna muricata L.) and investigate their potency as chemopreventive agents. Research steps include (1) identify the active compounds using thin-layer chromatography (TLC); (2) find out the potential compounds against cancer cells by molecular docking using Autodock Vina; (3) conduct a potential antioxidant test using free radicals DPPH (1,1-diphenyl-2-pikrihidrazil); and (4) identify the cytotoxic effect on WiDr colon cancer cells test using MTT Assay method. The results showed that the ethanolic extract of Camellia sinensis-Annona muricata leaf combination was suspected of containing flavonoid compounds with Rf values of 0.66 and 0.68. Besides, the DPPH antioxidant test showed an IC50 value of 26.9 μg/mL. Cytotoxic potential against WiDr cells resulted in an IC50 value of 41 μg/mL. Furthermore, the molecular docking test of epigallocatechin gallate (EGCG) and Acetogenin compounds against bcl-xl target proteins showed the docking score of -8.1 kcal/mol and -6.7 kcal/mol, respectively. It concluded that the extract combination of Camelia Sinensis-Annona muricata leaf had strong potency as an antioxidant and chemopreventive agent against the WiDr colon cancer cells line.
References
NCI. Cancer Treatment. 2017. Available from: http://www.cancer.gov/cancertopics/treatment.html. 2012. [cited 2017 May 9]
IARC. Tumours of the Colon and Rectum. 2012 [cited 2017 May 9]. https://www.iarc.fr/en/publications/pdfs-online/pa.
Aziz M, Andrijono, Saifuddin, A B. Buku Acuan Nasional Onkologi Ginekologi. Jakarta: Yayasan Bina Pustaka Sarwono Prawirohardjo. 2006.
AS T, AS M. Facing Cancer: a Complete Guide for People with Cancer, Their Families, and Caregivers (CLS Education). In: 1st ed. United States of America; 2004: 2–3.
Yuhernita, Juniarti. Analisis Senyawa Metabolit Sekunder dari Ekstrak Metanol Daun Surian yang Berpotensi sebagai Antioksidan. MAKARA Sci Ser. 2011; 15(1): 48–52. https://doi/org/10.7454/mss.v15i1.877
Alamansyah A. Taklukkan Penyakit dengan Teh Hijau. Jakarta: Agro Media Pustaka; 2006: 34–36.
Fulder S. Khasiat Teh Hijau in 1st ed., Jakarta: Prestasi Pustaka. 2004.
Wulan J. Dahsyatnya Khasiat Sirsak untuk Banyak Penyakit yang Mematikan. Yogyakarta: ANDI. 2012.
Prayoga E. Perbandingan Efek Ekstrak Daun Sirih Hijau (Piper betle L.) dengan Metode Difusi Disk dan Sumuran terhadap Pertumbuhan Bakteri Staphylococcus aureus. Skripsi. UIN Syarif Hidayatullah. 2013.
Mardawati E, Achyar C, Marta H. Kajian Aktivitas Antioksidan Ekstrak Kulit Manggis (Garcinia mangostana L) dalam Rangka Pemanfaatan Limbah Kulit Manggis di Kecamatan Puspahiang Kabupaten Tasikmalaya. Teknotan J Ind Teknol Pertanian. 2008; 2(3).
Motiejunas D, Wade RC. Structural, Energetic, and Dynamic Aspects of Ligand-receptor Interactions. Compr Med Chem II. 2006; 4:193–214.
Mukesh B, Rakesh K. Molecular Docking: A Review. Int J Res Ayurveda Pharm. 2011; 2(6): 1746–1751.
Sang JJ, Dong HH, Jeong HK. Inhibition of Proliferation and Induction of Apoptosis by EGCG in Human Osteogenic Sarcoma (HOS) Cells. Arch Pharm Res. 2006; 29(5): 363–368.
Noda C, He J, Takano T, Tanaka C, Kondo T, Tohyama K, et al. Induction of apoptosis by epigallocatechin-3-gallate in human lymphoblastoid B cells. Biochem Biophys Res Commun. 2007;362(4):951–957. https://doi.org/10.1016/j.bbrc.2007.08.079
Nakazato T, Ito K, Miyakawa Y, Kinjo K, Hozumi N, Ikeda Y, et al. Catechin, a green tea component, rapidly induces apoptosis of myeloid leukemic cells via modulation of reactive oxygen species production in vitro and inhibits tumor growth in vivo. Haematologica. 2005;90(3):317–325.
Nihal M, Ahmad N, Mukhtar H, Wood GS. Anti-proliferative and proapoptotic effects of (-)-epigallocatechin-3- gallate on human melanoma: Possible implications for the chemoprevention of melanoma. Int J Cancer. 2005;114(4):513–521. https://doi.org/10.1002/ijc.20785
Li HC, Yashiki S, Sonoda J, Lou H, Ghosh SK, Byrnes JJ, et al. Green tea polyphenols induce apoptosis in vitro in peripheral blood T lymphocytes of adult T-cell leukemia patients. Japanese J Cancer Res. 2000;91(1):34–40 https://doi.org/10.1111/j.1349-7006.2000.tb00857.x
Lee JH, Jeong YJ, Lee SW, Kim D, Oh SJ, Lim HS, et al. EGCG Induces Apoptosis in Human Laryngeal Epidermoid Carcinoma Hep2 Cells via Mitochondria with the Release of Apoptosis-inducing Factor and Endonuclease G. Cancer Lett. 2010; 290(1): 68–75. https://doi.org/10.1016/j.canlet.2009.08.027
Vergote D, Cren-Olivé C, Chopin V, Toillon RA, Rolando C, Hondermarck H, et al. (-)-Epigallocatechin (EGC) of Green Tea Induces Apoptosis of Human Breast Cancer Cells but not of Their Normal Counterparts. Breast Cancer Res Treat. 2002; 76(3): 195–201. https://doi.org/10.1023/a:1020833410523
Kavanagh KT, Hafer LJ, Kim DW, Mann KK, Sherr DH, Rogers AE, et al. Green Tea Extracts Decrease Carcinogen-induced Mammary Tumor Burden in Rats and Rate of Breast Cancer Cell Proliferation in Culture. J Cell Biochem. 2001; 82(3): 387–398. https://doi.org/10.1002/jcb.1164
Lin JK, Liang YC, Lin-Shiau SY. Cancer Chemoprevention by Tea Polyphenols through Mitotic Signal Transduction Blockade. Biochem Pharmacol. 1999; 58(6): 911–5.
Zhou B, Pan J, Dai F, Zhao C, Zhang L, Wei Q, et al. Redifferentiation of Human Hepatoma Cells Induced by Green Tea Polyphenols. Res Chem Intermed. 2004; 30(6): 627–36.
Aminah S, Pramono S. Isolasi Flavonoid Daun Murbei (Morus Alba L.) serta Uji Aktivitasnya sebagai Penurun Tekanan Darah Arteri pada Anjing Teranestesi. Maj Farm. 2017; 9(1): 235–42. https://doi.org/10.22146/farmaseutik.v9i1.24103
Suhendi A, Sjahid R, Hanwar D. Isolasi dan Identifikasi Flavonoid dari Daun Dewandaru (Eugenia uniflora L.) Isolation and Identification of Flavonoids from Dewandaru (Eugenia uniflora L.) Leaf. 73 Pharmacon. 2011; 12(2): 73–81. https://doi.org/10.23917/pharmacon.v12i2.36
Neldawati, Ratnawulan, Gusned. Analisis Nilai Absorbansi dalam Penentuan Kadar Flavonoid untuk Berbagai Jenis Daun Tanaman Obat. Pillar of Physics,. 2013; 2: 76–83.
Andersen O, Markham K. Flavonoids: Chemistry, Biochemistry, and Applications. CRC, Taylor & Francis, Boca Raton, FL; 2006.
Downloads
Published
Issue
Section
License
Copyright
Authors retain copyright and grant Mutiara Medika: Jurnal Kedokteran dan Kesehatan (MMJKK) the right of first publication with the work simultaneously licensed under an Attribution 4.0 International (CC BY 4.0) that allows others to remix, adapt and build upon the work with an acknowledgment of the work's authorship and of the initial publication in Mutiara Medika: Jurnal Kedokteran dan Kesehatan (MMJKK).
Authors are permitted to copy and redistribute the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Mutiara Medika: Jurnal Kedokteran dan Kesehatan (MMJKK).
License
Articles published in the Mutiara Medika: Jurnal Kedokteran dan Kesehatan (MMJKK) are licensed under an Attribution 4.0 International (CC BY 4.0) license. You are free to:
- Share — copy and redistribute the material in any medium or format.
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
This license is acceptable for Free Cultural Works. The licensor cannot revoke these freedoms as long as you follow the license terms. Under the following terms:
Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
