Efek Antiinflamasi Daging Buah Mahkota Dewa (Phaleria macrocarpa [Scheff.] Boerl) pada Tikus Betina Terinduksi Karagenin
DOI:
https://doi.org/10.18196/mmjkk.v10i2.1577Keywords:
edema, inflamasi, karagenin, Phaleria macrocarpa, carrageenan, inflammationAbstract
The objective of the research is to understand the anti-inflammation effect of Mahkota Dewa fruits (Phaleria macrocarpa) in the female rats foot edema induced by carrageenan. Research design is a simple randomized experimental. Samples taken a number of 30 female mice of Wistar groove, divided into 5 groups randomly, each group consisted of 6 mice. All groups of mice fasted for 12 hours. Volume measurement is done early by pletismograph for 3 times. Mice were given treatment orally with aquades, ibuprofen, infusa of mahkota dewa 2.1%, 4.2%, and 8.4%. One hour later the right foot injected with carrageenan 1% subplantar. After injection the right foot volume measured as the volume of minutes 0 (T0) further measurements made every 15 minutes to the minutes 240 (T16). Anti-Inflammation effect votes average difference with the volume of foot edema. Data statistically tested with Anova and LSD type Post Hoc Analysis-Multiple Comparison. The result shown that every mahkota dewa doses have different effect significantly with negative controlp<0,005. Doses group with 2,1% and 4,2% different not significantly with positive controlp>0,05. Dose 8,4% different significanly both. It show that mahkota dewa have anti-inflammation effect.
Tujuan penelitian ini adalah untuk mengetahui efek antiinflamasi daging buah mahkota dewa (Phaleria macrocarpa) pada edema kaki tikus betina terinduksi karagenin. Disain penelitian adalah eksperimental acak sederhana. Sampel 30 ekor tikus betina galur Wistar, dibagi 5 kelompok secara acak, masing-masing kelompok terdiri dari 6 ekor tikus. Seluruh kelompok tikus dipuasakan selama 12 jam. Volume awal kaki kanan tikus diukur dengan pletismograph sebanyak 3 kali. Tikus diberi perlakuan peroral berupa aquades, ibuprofen, infusa mahkota dewa 2,1%, 4,2%, dan 8,4%. Satu jam kemudian telapak kaki kanan tikus disuntik karagenin 1% subplantar. Setelah penyuntikan volume kaki kanan diukur sebagai volume menit ke-0 (T0), dilakukan pengukuran tiap 15 menit hingga menit ke-240 (T16). Daya inflamasi dinilai dengan menghitung rerata selisih volume edema kaki. Data diuji secara statistik dengan Anova dan Post Hoc Analysis - Multiple Comparison tipe LSD. Hasil penelitian menunjukkan bahwa masing-masing dosis mahkota dewa mempunyai efek berbeda signifikan terhadap kontrol negatif p<0,05. Kelompok dosis 2,1% dan 4,2% berbeda tidak bermakna dibandingkan kontrol positif p>0,05. Kelompok dosis 8,4% perbedaan bermakna baik kontrol positif maupun kontrol negatif. Ini membuktikan bahwa mahkota dewa memiliki efek antiinflamasi.
References
Soenarto. 2006. Inflamasi. In Sudoyo A.W., Setiyohadi B., Alwi I., Simadibrata M., Setiati S. (Eds.). Buku Ajar Ilmu Penyakit Dalam (4th Ed.). Jakarta: Departemen Ilmu Penyakit Dalam. Fakultas Kedokteran Universitas Indonesia.
Larocco, M. 1994. Pathophysiology Concepts of Altered Health States (4th Ed.). Philadelphia: J.B. Lippincott Company. P 243-251.
Punchard, N.A., Whelan, C.J., & Adcock. I. 2004. 27 September. The Journal of Inflammation. BioMed Central. Diakses 21 April 2008, dari http://www.journal- inflammation.com/content/l/l/l
Inflammation, 2008, 28 April. Diakses 29 April 2008, dari http://en.wikipedia .org/wiki/Inflammation
Burke, A., Smyth, E., & Fitzgerald, G.A. 2006. Analgesic-Antipyretic and Antiinflammatory Agents; Pharmacoterapy of Gout. In Laurence. L. Brunton, John.S.L., Keith L. P. (Eds.), Goodman & Gilman’s, The Pharmacological Basis of Therapeutics (11th Ed.). New York. The McGraw-Hill Companies, Inc. P 671-701.
Hadisoewignyo, L. & Fudholi, A. 2007. Studi Pelepasan In Vitro Ibuprofen dari Matriks Xanthan Gum yang Dikombinasikam dengan Suatu Crosslinking Agent. Majalah Farmasi Indonesia. 18(3), 133-140.
Harmanto, N. 2001. Seri Agrisehat Mahkota Dewa Obat Pusaka Para Dewa. Jakarta: Agromedia Pustaka.
Winarto, W.P. dan Tim Karyasari. 2003. Seri Agrisehat, Mahkota Dewa, Budi Daya dan Pemanfaatan untuk Obat. Jakarta: Penebar Swadaya.
Mahkota Dewa Nusantara. 2005. Diakses 30 Maret 2008, dari http://www. geocities.com/lanklax/mahkotadewa/
Kim, H.P., Son, K.H., Chang, H.W., & Kang, S.S. 2004. Antiinflammatory Plant Flavonoids and Cellular Action Mechanisms. Journal of Pharmacological Sciences. 96, 229¬245
Vinegar R., Truax, J.F., & Selph, J.L. 1976. Quantitative Studies of The Pathway to Acute Carrageenan Inflammation [Abstrak]. Fed proc. 35(13), 2447-2456.
Mariani, R. 2005. Telaah Kandungan Kimia dan Aktivitas Antiradang Buah Mahkota Dewa (Phaleria Macrocarpa [Scheff.] Boerl [Abstrak]. Disertasi Doktor. Institut Teknologi Bandung, Bandung.
Downloads
Published
Issue
Section
License
Copyright
Authors retain copyright and grant Mutiara Medika: Jurnal Kedokteran dan Kesehatan (MMJKK) the right of first publication with the work simultaneously licensed under an Attribution 4.0 International (CC BY 4.0) that allows others to remix, adapt and build upon the work with an acknowledgment of the work's authorship and of the initial publication in Mutiara Medika: Jurnal Kedokteran dan Kesehatan (MMJKK).
Authors are permitted to copy and redistribute the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Mutiara Medika: Jurnal Kedokteran dan Kesehatan (MMJKK).
License
Articles published in the Mutiara Medika: Jurnal Kedokteran dan Kesehatan (MMJKK) are licensed under an Attribution 4.0 International (CC BY 4.0) license. You are free to:
- Share — copy and redistribute the material in any medium or format.
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
This license is acceptable for Free Cultural Works. The licensor cannot revoke these freedoms as long as you follow the license terms. Under the following terms:
Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
