Perbedaan Prevalensi Toksoplasmosis pada Tikus dengan Uji Serologis Metode ELISA di Kecamatan Wirobrajan dan Sekitarnya
DOI:
https://doi.org/10.18196/mmjkk.v9i1%20(s).1618Keywords:
ELISA, Habitat, Prevalensi, toksoplasmosis, uji serologis, habitat, prevalence, serologic test, ToxoplasmosisAbstract
Toksoplasmosis in mouse, though it doesn ’t infect directly to human, it is indirectly the source of infection to human. This is so because mouse is animal hunted by cat. Every mouse has different characteristic in every habitat, ranging from the side of ecosystem, kind offeed consumed, and how many mice can interact with other hospes like cat. From this habit it can result in the difference incidence of mouse infected by toxosoplasmosis and what extent it can infect other hospes based on its habitat. This research was to find out toxoplasmosis prevalence at mouse based on habitat serologically with ELISA method in Kecamatan Wirobrajan and the surroundings. This research used observasional method. Sample from this research was all mice caught in areas of river, market, and houses in Kecamatan Wirobrajan and the surroundings. Data collected were from mice as many as 83 mice namely 34 mice from market area, 23 mice from river area, and 26 mice from the housing area, which were taken the serum then it was read using ELISA. The result from ELISA showed that 4 mice (4.8%) were positively infected by toxoplasmosis, namely 1 mouse from the market (1.2%), 3 mice (3.6%) from the house, and found no mouse that was positive from the river (0%), then the result was examined statistically using the Kruskal Walis test that indicated the value of p=0,14 (P>0,005). Based on the result, it could be concluded that the difference of toksoplasmosis prevalence at mouse based on its habitat was not significantly different.
Toksoplasmosis pada tikus meskipun tidak dapat menular secara langsung pada manusia, tetapi secara tidak langsung merupakan sumber penularan kepada manusia. Hal ini disebabkan oleh karena tikus merupakan binatang buruan bagi kucing. Setiap tikus memiliki karakteristik yang berbeda- beda di setiap habitatnya, mulai dari segi tempat hidupnya, jenis makanan yang dikonsumsinya, dan seberapa banyak tikus itu dapat berinteraksi dengan hospes lainnya seperti kucing. Dari kebiasaan inilah dapat saja menimbulkan perbedaan insidensi tikus yang terinfeksi toksoplasma dan sejauh mana dapat menginfeksi hospes lain berdasarkan habitatnya tersebut. Penelitian ini bertujuan untuk mengetahui prevalensi Toksoplasmosis pada tikus berdasarkan habitat secara serologis dengan metode ELISA di Kecamatan Wirobrajan dan sekitarnya. Penelitian menggunakan metode observasional. Sampel penelitian adalah semua tikus yang tertangkap di sungai, pasar, dan perumahan di Kecamatan Wirobrajan dan sekitarnya. Data terkumpul yaitu tikus sebanyak 83 ekor, 34 ekor berasal dari pasar, 23 ekor dari sungai, dan 26 ekor berasal dari perumahan, diambil serum darah lalu dibaca dengan ELISA. Hasil ELISA diperoleh 4 ekor tikus (4.8%) positif terkena toksoplasma yaitu, 1 ekor berasal dari pasar (1.2%), 3 ekor (3.6%) berasal dari rumah, dan tidak ditemukan satupun tikus yang positif yang berasal dari sungai (0%), uji statistik dengan metode uji kruskal walis menunjukkan nilai p=0,14 (P>0,005). Berdasarkan hasil tersebut dapat disimpulkan bahwa perbedaan prevalensi toksoplasmosis pada tikus berdasarkan habitatnya tidak berbeda bermakna.
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