Uji Potensi Antibakteri Sodium Ascorbyl Phosphate terhadap Propionibacterium acnes In Vitro
DOI:
https://doi.org/10.18196/mmjkk.v11i1.930Keywords:
Akne vulgaris, Propionibacterium acnes, Sodium Ascorbyl Phosphate, kadar hambat minimalAbstract
Akne vulgaris adalah gangguan pada unit pilosebaseus yang sering terjadi pada dewasa muda. Propionibacterium acnes adalah bakteri anaerob yang merupakan flora folikel dan berperan penting dalam patogenesis akne. Sodium Ascorbyl Phosphate (SAP) merupakan bentuk phosphate ester ascorbic acid yang mudah diserap oleh kulit, sehingga sering digunakan dalam produk kosmetik baik sebagai senyawa tunggal maupun campuran. Apabila senyawa ini terbukti mempunyai pengaruh terhadap jumlah koloni P. acnes, maka SAP dapat digunakan sebagai salah satu obat alternatif antiakne. Tujuan penelitian adalah untuk mengetahui kadar minimal SAP yang dapat menghambat pertumbuhan P. acnes invitro. Penelitian dilakukan secara eksperimental lab dengan bahan uji SAP yang dibuat dalam konsentrasi 0,5%, 1%, 1,5%, 2%, 2,5 %, 3%, 3,5%, 4%, 4,5 % kadar hambat minimal SAP terhadap P. acnes dengan metode dilusi cair. Penelitian ini dilakukan pengulangan sebanyak 3 kali. P. acnes cair di dapatkan dari Laboratorium Mikrobiologi FK UGM. Hasil penelitian menunjukkan bahwa kadar hambat minimal SAP terhadap Propionibacterium acnes adalah 2,5 % b/v. Dari penelitian ini dapat disimpulkan bahwa SAP memiliki potensi antibakteri terhadap Propionibacterium acnes.
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