Daya Hambat Ekstrak Etanol Aloe Vera L. terhadap Proliferasi Sel Kanker Rongga Mulut (Sp-C1) secara In Vitro
DOI:
https://doi.org/10.18196/mmjkk.v12i1.996Keywords:
Aloe vera, proliferasi kanker lidah, In Vitro, proliferation, oral tongue carcinoma, SP-C1Abstract
Kanker adalah penyakit yang ditandai dengan pembelahan sel yang tidak terkendali dan kemampuan sel-sel tersebut untuk menyerang jaringan biologis lainya, Pada penelitian ini menggunakan Lidah Buaya (Aloe vera L) yang memiliki banyak khasiat sebagai anti kanker, anti bakteri, anti jamur, anti inflamasi, dan memiliki efek analgesik. Tujuan dari penelitian ini adalah mengkaji daya hambat proliferasi sel kanker rongga mulut (SP-C1) menggunakan ekstrak etanol Lidah buaya. Desain penelitian ini adalah eksperimental laboratoris murni. Subjek penelitian pada penelitian ini menggunakan kultur sel kanker lidah (SP-C1) yang dibiakan dalam media Dubelcco’s modified eagle medium (DMEM) yang diberi foetal Bovine Serum 10% (FBS). JenisLidah buaya yang digunakan pada penelitian ini adalah Aloe vera L arborescens. Sel SP-C1 yang tumbuh sub-confluent dipanen menggunakan Tripsin-EDTA 0,25%. Sel sebanyak 1x 104 sel/sumur dimasukkkan cawan petri 24 sumur, sesuai jumlah dengan konsentrasi ekstrak etanol Lidah buaya yang digunakan. Sel di inkubasi selama 24 jam. Setelah inkubasi, semua media dibuang dan diganti dengan media baru yang mengandung berbagai konsentrasi ekstrak Lidah buaya. Sel di inkubasi selama 0, 24, 48 dan 72 jam. Hasil Penelitian menunjukkan pemberian ekstrak etanol Lidah buaya pada konsentrasi 75 mg/ml dan 100 ml/mg cenderung menurunkan jumlah proliferasi sel SP-C1 dibandingkan dengan konsentrasi 0 mg/ml, 25 mg/ml, dan 50 mg/ml dengan signifikansi p<0.05. Kesimpulan: Ekstrak etanol Lidah buaya efektif menghambat proliferasi sel kanker rongga mulut SP-C1 secara In Vitro.
Cancer is a desease that have characterized uncontrolled mitosis and invasion to the other organs. In this researched used Aloe vera as alternative herbal to cure cancer. Aloe vera contains are anticancer, antifungi, antiinflamation and analgesic. The aim of this research is to know the effect of etanolic extract of Aloe vera in inhihibit proliferation of SP-C1. Design research is pure laboratory experimental. Subject is a culture SP-C1 cells in Dubelcco’s modified eagle medium (DMEM) and foetal Bovine Serum 10% (FBS). The kind of Aloe vera is Aloe vera L arborescens. The Fluectuent SP-C1 cell collect by Tripsin-EDTA 0,25%. Cell put in cawan petri 24 cell/well with etanolic extract of Aloe vera, incubation 24 hours and replace with new media, incubation in 24, 48 and 72 hours. Each group add with MTT solution and counted by ELISA. The result of this research shows that concentration 75 mg/ml and 100 mg/ml effective inhibit proliferation of SP-C1 cell compared with 0 mg/ml, 25 mg/ml and 50 mg/ml with the value of significant level 0.05.
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