Prevalence of Dengue Virus Transovarial Transmission and DHF Incidence Rate in Grogol Sub-district of Sukoharjo District

Paul Sunardi, Hari Kusnanto, Tri Baskoro T. Satoto, Lutfan Lazuardi


Environmental changes in physical, biological, and social aspects have enabled the occurrence of DHF cases in Sukoharjo. Such changes may affect virulence of dengue virus, shorten extrinsic periods and increase vector dengue capacity. This study aimed to determine the spread of dengue virus transovarial transmission and the incidence rate of DHF in Grogol Sub-district of Sukoharjo District. This observational analytic study used a cross-sectional approach conducted in 2016. The population was Ae. aegypti mosquitoes and DHF patients (based on laboratory diagnosis with serologic examination, i.e., IgG, IgM and/or NS1 indicating positive DHF). Data analysis used Chi-square, Multiple Linear Regression, and spatially weighted regression with GeoDa and SatScan. The study found virDEN-3 transovarial transmission in Grogol Sub-district of Sukoharjo District and the spread of virDEN-3 transovarial transmission following the high spread of Ovitrap Index. The DHF incidences (cases) tended to cluster at a radius of 100-200 m from a region found the positive virDEN-3 transovarial transmission. The statistical tests showed a relationship between the virDEN-3 transovarial transmission and the DHF incidence with a p-value of <0.05. Spatially, the incidence of DHF in this area followed a certain spatial distribution pattern, with a clustering of dengue cases centered on the coordinates of -7.588240 S, 110.809450 E with a radius of 2.00 km.


DHF; virDEN-3; Transmission; Mosquitoes; Virus;

Full Text:



WHO. 2004. Situation Of Dengue/Dengue Haemorrhagic Fever In the South-East Asia Region: Prevention And Control Status In SEA Countries [Internet]. Terdapat dalam: [Retrieved August 27, 2014].

Henchal, E. A dan Putnak, J.R. 1990. The Dengue viruses. Clin Microbiol Rev : 3 (4) : 376-396.

Bai, L., Xia, S., Lan, R., Liu, L., Ye, C., Wang, Y., et al, 2013. Isolation and Characterization of cytotoxic Aggregative Citrobacter freundii. (N. Ahmed, Ed.) Plos One, 7(3), 1-2.

Mardihusodo, S.J., Satoto, T.B.T., Mulyaningsih, B., Umniyati, S.R. & Ernaningsih. 2007. Evidence of Transmission of Transmissal Dengue Virus In Aedes aegypti Mosquito In Yogyakarta City. National Symposium on Molecular Biology Aspect, Pathogenesis, Management and Prevention of Outbreak, Center for Biotechnology Studies UGM, Yogyakarta, 16 May 2007.

Tri Boewono, Widiarti, Umi Widyastuti, Mujiono & Lasmiati, 2006. Detection of Dengue virus in the parent and progeny of Aedes aegypti dengue vector in some endemic areas in Central Java. In one day seminar vector control and disease reservoir strategy on emergency disaster emergency in the era of decentralization, Salatiga 20 September 2006.

Perich, M.J, Kardec. A, Braga, I.A., Portal, L.F., Burge, R, Zeichner, B.C, Brogdon, W.A., Wirtz, R.A., 2003. Field Evaluation of a Lethal Ovitrap Against DenguenVector in Brazil, Medical Veterinary Entomology, Brazil, 205-209,, Download 05 Maret 2007.

Hasyimi, M., Eny W. Lestari, Supratman, S. 1992. Pleasure Spawning Aedes sp, Center for Health Ecology Research, Research and Development Agency of Health MOH RI, Jakarta, delivered to Congress Entomologi IV, in Yogyakarta.

Mardihusodo S.J. 1993. Aedes aegypti Resistance Early Detection Research Report on Malathion and Temephos. Research Institute of Gadjah Mada University, Yogyakarta.

Sudjadi, 2008. Health Biotechnology. Kanisius Publisher. Yogyakarta. Hal: 94-99 and stuff: 131-143

Harris,E. T.G.Robert. L.Smith. al. 1998. Typing of Dengue Viruses in Clinical Specimens and Mosquitoes by Single-Tube Multiplex Reverse Transcriptase PCR, Journal of Clinical Microbiology, Sept.1998,p.2634- 2639

Charoenpanyanet, A and Chen, X. 2008. Satellite-Based Modeling of Anopheles Mosquito Densities on Heterogeneous Land Cover in Western Thailand. The International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences. Vol. XXXVII. Part B8. Beijing.

Diggle, P., 2003. Statistical Analysis of Spatial Point Patterns, 2nd ed. Arnold, London, UK.

Joshi V, Mourya DT, Sharma RC, 2002. Persistence of Dengue-3 Virus Through Transovarial Transmission Passage In Successive Generations of Aedes aegypti Mosquitoes. Am.J.Trop.Med.Hyg.,67 :158 –161.

Thu,H.M., K. Lowry,T.T.Myint,et al. 2004. Myanmar Dengue Outbreak Associated with Displacement of Serotype 2,3, and 4 by Dengue 1. Emerg. Infection.10:593-597

Lee, H.L. & Rohani, A. 2005. Transovarial Transmission Of Dengue Virus In Aedes aegypti And Aedes albopictus In Relation to Dengue Outbreak In An Urban Area In Malaysia. Dengue Bulletin. 29 : 106-111.


Article Metrics

Abstract view : 37 times
PDF - 25 times


  • There are currently no refbacks.

Copyright (c) 2018 JMMR (Jurnal Medicoeticolegal dan Manajemen Rumah Sakit)

JMMR (Jurnal Medicoeticolegal dan Manajemen Rumah Sakit) are indexed by:


Ruang Jurnal JMMR, Gedung Pascasarjana Universitas Muhammadiyah Yogyakarta
Brawijaya Street, Tamantirto, Kasihan, Bantul, D.I. Yogyakarta, Indonesia

Information for Author click HERE

Creative Commons License
JMMR is licensed under Creative Commons Attribution 4.0 International License.

View My Journal of JMMR