Antioxidant Activity and Vitamin C Concentration Analysis of Gandaria (Bouae macrophylla Griff) Ethanol Extract Using Spectrophotometry UV Vis
DOI:
https://doi.org/10.18196/jfaps.v3i2.15992Keywords:
antioxidants, gandaria fruit, spectrophotometry UV-Vis, vitamin CAbstract
Antioxidants are chemical compounds that donate one or more electrons to free radicals to inhibit free radical reactions. One of the potential antioxidant sources is gandaria fruit (Bouea Macrophylla Griff). This research aims to determine the antioxidant activity and vitamin C content of the gandaria fruit extract. Gandaria fruit was macerated with ethanol as a solvent. Furthermore, the rotated extract was carried out for the phytochemical screening. The results of the phytochemical screening of the gandaria fruit extract showed a positive of flavonoids, terpenoids, tannins, saponins, and phenolics. The gandaria fruit extract was tested for antioxidants and quantitative analysis of vitamin C levels to obtain IC50 values and vitamin C levels using spectrophotometry UV-Vis at a wavelength of 517nm with vitamin C as a positive control. The results of spectrophotometric measurements revealed that the gandaria fruit extract had an IC50 value of 5.72 g/ml, and vitamin C had an IC50 value of 2.260 g/ml, indicating that the gandaria fruit extract and vitamin C had very strong antioxidants. The value of vitamin C levels was 0.526 mg.References
Hanifa D, Susilawati Y. Potensi Tanaman Gandaria (Bouea macrophylla Griff) Sebagai Obat Herbal Yang Beraktivitas Antioksidan. Farmaka. 2017; 15(3): 134-142.
Kurniawan A. Uji Aktivitas Antioksidan Menggunakan Radikal 1,1-Difenil-2-Pikrilhidrazil (Dpph) Dan Penetapan Kandungan Fenolik Total Fraksi Etil Asetat Ekstrak Etanolik Herba Seledri. Skripsi thesis, Sanata Dharma University. 2011.
Harsono, T. Tinjauan Ekologi Dan Etnobotani Gandaria (Bouea Macrophylla Griffith). Jurnal Biosains, 2017; 3(2), 119. https://doi.org/10.24114/jbio.v3i2.7584
Cahyani AI. Uji Aktivitas Antioksidan Dari Ekstrak Kulit Batang Kayu Jawa ( Lannea Coromandelica ) Dengan Metode Dpph (2,2-Difenil-1-Pikrilhidrazil). Skripsi. UIN Syarif Hidayatullah Jakarta. 2017.
Londo N. Bioaktivitas Ekstrak Kasar Biji Gandaria Bouea Macrophylla Griff Sebagai Bahan Antioksidan. Thesis, 53(November)2015; 1689–1699.
Muflihunna A, Sarif LM. Analisis Aktivitas Antioksidan Produk Sirup Buah Mengkudu ( Morinda Citrifolia L .) Dengan Metode Dpph. 2016; 2(2), 97–101. https://doi.org/10.33096/jffi.v2i2.177
Rinanda R. Penetapan Kadar Vitamin C Dan Uji Aktivitas Antioksidan Pada Cabai Rawit Hijau (Capsicum Frutescens L.). Thesis. Universitas Perintis Indonesia. 2019.
Jayantie DD, Farida Y, Taurhesia S. Pharmacoscript. 2022; 5(1): 62–70. https://doi.org/10.36423/pharmacoscript.v5i1.856
Budilaksono W, Wahdaningsih S, Fahrurroji A. Uji Aktivitas Antioksidan Fraksi N-Heksana Kulit Buah Naga Merah (Hylocereus Lemairei Britton Dan Rose) Menggunakan Metode Dpph (1,1-Difenil-2-Pikrilhidrazil). Jurnal Mahasiswa Farmasi Fakultas Kedokteran UNTAN. 2014; 1(1). https://doi.org/10.26418/jurkeswa.v1i2.42997
Dewi AP. Penetapan Kadar Vitamin C Dengan Spektrofotometri Uv-Vis Pada Berbagai Variasi Buah Tomat. JOPS. 2018; 2(1): 9-13 https://doi.org/10.36341/jops.v2i1.1015
Fatmawati S. Pengaruh Perbedaan Metode Ekstraksi Maserasi Dan Perkolasi Terhadap Uji Aktivitas Antioksidan Ekstrak Kulit Buah Naga Merah (Hylocereus Polyrhizus). Politeknik Harapan Bersama. Tegal. 2019
Helwandi, Ika R. Validasi Metode Spektrofotometri Uv-Vis Analisis Tiga Panjang Gelombang Untuk Penetapan Kadar Tablet Prednison Yang Mengandung Zat Pewarna. 2016.
Khairunnisa N. Uji Aktivitas Antioksidan Pada Ekstrak Daun Zaitun (Olea Europaea L.) Menggunakan Pelarut Air Dengan Metode Dpph. Skripsi Universitas Islam Negeri Syarif Hidayatullah, 2019; 1–62.
Lolaen LAC, Fatimawali F, Citraningtyas G. Uji Aktivitas Antioksidan Kandungan Fitokimia Jus Buah Gandaria ( Bouea Macrophylla Griffith). Pharmacon. 2013; 2(2): 1–8.
Murwanti R, Rahmadani A, Kholifah E, Hermawan A. Effect Of Curcumin On Mmp-9 Activity Of 4t1 Murine Triple-Negative Breast Cancer Cells Effect Of Curcumin On Mmp-9 Activity Of 4t1 Murine Triple-Negative Breast Cancer Cells. In AIP Conference Proceedings 2020; 2260(1): 060017). https://doi.org/10.1063/5.0016424
Rusmiati. Pengaruh Metode Ekstrak Terhadap Aktivitas Antimikroba Ekstrak Metanol Daun Mimba. Skripsi. Universitas Islam Negeri Alauddin Makassar. 2010.
