Phytochemical Analysis and Antioxidant Potential of Ethylacetate Extract of Tamarindus Indica (Tamarind) Leaves by Frap Assay

Mubarak Muhammad Dahiru, Hadiza Ahmadi, Maimuna Umar Faruk, Huzaifa Huzaifa Aminu Hamman, Abreme Gahana Charles

Abstract


Oxidative stress is characterized by an imbalance in the generation of free radicals and their subsequent elimination by endogenous antioxidants. It is a characteristic of several diseases, especially during the progression stage, which can lead to fatal effects. This study aims to investigate the phytochemical components and antioxidant capability of Tamarindus indica and assess its capability as a candidate for managing diseases associated with oxidative stress. The gravimetric method detected and quantified phytochemicals, while the reducing power assay determined the antioxidant potential. Saponins, steroids, and flavonoids were detected in 6.83 ±0.44, 4.30 ±0.60, and 10.17% ±0.60, respectively, without alkaloids, glycosides, and terpenoids. The antioxidant test showed a concentration-dependent increase in absorbance of both the extract and standard (Ascorbic acid). However, Ascorbic acid had higher absorbance. At 100% concentration, the sample had an absorbance of 0.388 ±0.022, which was lower than the absorbance of Ascorbic acid (0.411 ±0.009) at 40% concentration. It can be concluded that Tamarind leaves could be utilized to manage diseases associated with oxidative stress, evidenced by their antioxidant potential credited to the phytochemical content of the leaves. However, there is a need for further studies to ascertain the exact compounds and their modes of action.


Keywords


antioxidant; ethylacetate; oxidative stress; phytochemical; tamarindus indica

Full Text:

PDF

References


Sies H. Oxidative stress: a concept in redox biology and medicine. Redox biology. 2015;4:180-3.

Hayes JD, Dinkova-Kostova AT, Tew KD. Oxidative Stress in Cancer. Cancer Cell. 2020 ;38(2):167-97.

Salim S. Oxidative Stress and the Central Nervous System. J Pharmacol Exp Ther. 2017;360(1):201.

Bhatti JS, Sehrawat A, Mishra J, Sidhu IS, Navik U, Khullar N, et al. Oxidative stress in the pathophysiology of type 2 diabetes and related complications: Current therapeutics strategies and future perspectives. Free Radical Biol Med. 2022.

Pisoschi AM, Pop A. The role of antioxidants in the chemistry of oxidative stress: A review. European Journal of Medicinal Chemistry. 2015 ;97:55-74.

Tsapralis D, Panayiotides I, Peros G, Liakakos T, Karamitopoulou E. Human epidermal growth factor receptor-2 gene amplification in gastric cancer using tissue microarray technology. World journal of gastroenterology: WJG. 2012;18(2):150.

Adwas AA, Elsayed A, Azab AE, Quwaydir FA. Oxidative stress and antioxidant mechanisms in human body. J Appl Biotechnol Bioeng. 2019;6(1):43-7.

Zhang P, Li T, Wu X, Nice EC, Huang C, Zhang Y. Oxidative stress and diabetes: antioxidative strategies. Frontiers of Medicine. 2020;14(5):583-600.

D’Oria R, Schipani R, Leonardini A, Natalicchio A, Perrini S, Cignarelli A, et al. The Role of Oxidative Stress in Cardiac Disease: From Physiological Response to Injury Factor. Oxidative Medicine and Cellular Longevity. 2020;2020:5732956.

Yaribeygi H, Panahi Y, Javadi B, Sahebkar A. The Underlying Role of Oxidative Stress in Neurodegeneration: A Mechanistic Review. CNS & Neurological Disorders - Drug Targets- CNS & Neurological Disorders). 2018;17(3):207-15.

Mileo AM, Miccadei S. Polyphenols as Modulator of Oxidative Stress in Cancer Disease: New Therapeutic Strategies. Oxidative Medicine and Cellular Longevity. 2016 ;2016:6475624.

Chang X, Zhang T, Zhang W, Zhao Z, Sun J. Natural drugs as a treatment strategy for cardiovascular disease through the regulation of oxidative stress. Oxidative Medicine and Cellular Longevity. 2020;2020.

Sarrafchi A, Bahmani M, Shirzad H, Rafieian-Kopaei M. Oxidative stress and Parkinson’s disease: New hopes in treatment with herbal antioxidants. Curr Pharm Des. 2016 ;22(2):238-46.

Chandran R, Abrahamse H. Identifying plant-based natural medicine against oxidative stress and neurodegenerative disorders. Oxidative Medicine and Cellular Longevity. 2020;2020.

Chikara S, Nagaprashantha LD, Singhal J, Horne D, Awasthi S, Singhal SS. Oxidative stress and dietary phytochemicals: Role in cancer chemoprevention and treatment. Cancer Lett. 2018 ;413:122-34.

Evans WC. Trease and Evans' pharmacognosy: Elsevier Health Sciences; 2009.

Obadoni B, Ochuko P. Phytochemical studies and comparative efficacy of the crude extracts of some haemostatic plants in Edo and Delta States of Nigeria. Global Journal of pure and applied sciences. 2002;8(2):203-8.

Harborne A. Phytochemical methods a guide to modern techniques of plant analysis: springer science & business media; 1998.

Oyaizu M. Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucosamine. The Japanese journal of nutrition and dietetics. 1986;44(6):307-15.

Mehdi MAH, Alarabi FY, Farooqui M, Pradhan V. Phytochemical screening and antiamebic studies of Tamarindus indica of leaves extract. Asian J Pharm Clin Res. 2019;12(2):507-12.

Adeniyi OV, Olaifa FE, Emikpe BO, Ogunbanwo ST. Phytochemical components and antibacterial activity of Tamarindus indica Linn. extracts against some pathogens. environment. 2017;7:14.

Ugoh SC, Jaruma IM. Phytochemical screening and antibacterial activity of the fruit and leaf extract of Tamarindus indica (Linn). Report and Opinion. 2013;5(8):18-27.

Chigurupati S, Eric WKY, Jahidul IM, Shantini V, Kesavanarayanan KS, Venkata RRM, et al. Screening antimicrobial potential for Malaysian originated Tamarindus indica ethanolic leaves extract. Asian J Pharm Clin Res. 2018;11(3).

Kagoro MPL, Muhammed BN, Auwal AA, Beskeni DR, Gongden JJ. PHYTOCHEMICAL SCREENING AND GC-MS ANALYSES OF A SUBFRACTION OF 70% ETHANOL EXTRACT OF Tamarindus indica (Linn.) Leaf. Journal of Chemical Society of Nigeria. 2022;47(1).

Herawati D, Pudjiastuti P. Effect of Different Solvents On The Phytochemical Compounds of Sargassum sp. From Yogyakarta and East Nusa Tenggara. Journal of Physics: Conference Series. 2021 ;1783(1):012001.

Cui Y, Liu B, Sun X, Li Z, Chen Y, Guo Z, et al. Protective effects of alfalfa saponins on oxidative stress-induced apoptotic cells. Food & function. 2020;11(9):8133-40.

Huang J-L, Jing X, Tian X, Qin M-C, Xu Z-H, Wu D-P, et al. Neuroprotective Properties of Panax notoginseng Saponins via Preventing Oxidative Stress Injury in SAMP8 Mice. Evidence-Based Complementary and Alternative Medicine. 2017 ;2017:8713561.

Wang M, Zhang X-J, Liu F, Hu Y, He C, Li P, et al. Saponins isolated from the leaves of Panax notoginseng protect against alcoholic liver injury via inhibiting ethanol-induced oxidative stress and gut-derived endotoxin-mediated inflammation. Journal of Functional Foods. 2015 ;19:214-24.

Zhang Y, Chi X, Wang Z, Bi S, Wang Y, Shi F, et al. Protective effects of Panax notoginseng saponins on PME-Induced nephrotoxicity in mice. Biomedicine & Pharmacotherapy. 2019;116:108970.

Diniz TC, Silva JC, Lima-Saraiva SRGd, Ribeiro FPRdA, Pacheco AGM, de Freitas RM, et al. The Role of Flavonoids on Oxidative Stress in Epilepsy. Oxidative Medicine and Cellular Longevity. 2015 ;2015:171756.

Zaidun NH, Thent ZC, Latiff AA. Combating oxidative stress disorders with citrus flavonoid: Naringenin. Life Sci. 2018;208:111-22.

Huyut Z, Beydemir Ş, Gülçin İ. Antioxidant and Antiradical Properties of Selected Flavonoids and Phenolic Compounds. Biochemistry Research International. 2017 ;2017:7616791.

Al-Numair KS, Chandramohan G, Veeramani C, Alsaif MA. Ameliorative effect of kaempferol, a flavonoid, on oxidative stress in streptozotocin-induced diabetic rats. Redox Report. 2015 ;20(5):198-209.

Cordero‐Herrera I, Martín MA, Goya L, Ramos S. Cocoa flavonoids protect hepatic cells against high‐glucose‐induced oxidative stress: Relevance of MAPKs. Mol Nutr Food Res. 2015; 59(4):597-609.

Chigurupati S, Yiik EWK, Vijayabalan S, Selvarajan KK, Alhowail A, Nanda SS, et al. Antioxidant and antidiabetic properties of Tamarindus indica leaf ethanolic extract from Malaysia. Southeast Asian Journal of Tropical Medicine and Public Health. 2020; 51(4):559-69.

Leng LY, binti Nadzri N, bin Shaari AR, Yee KC. Antioxidant capacity and total phenolic content of fresh, oven-dried and stir-fried tamarind leaves. Current Research in Nutrition and Food Science Journal. 2017; 5(3):282-7.

Amadou IM, Papa MG, Alioune DF, Modou OK, Kady DB, Abdou S, et al. Antioxidative activity of Tamarindus indica L. extract and chemical fractions. African Journal of Biochemistry Research. 2017; 11(2):6-11.

Nahar L, Nasrin F, Zahan R, Haque A, Haque E, Mosaddik A. Comparative study of antidiabetic activity of Cajanus cajan and Tamarindus indica in alloxan-induced diabetic mice with a reference to in vitro antioxidant activity. Pharmacognosy research. 2014 ;6(2):180.

Yeasmen N, Islam MN. Ethanol as a solvent and hot extraction technique preserved the antioxidant properties of tamarind (Tamarindus indica) seed. Journal of Advanced Veterinary and Animal Research. 2015 ;2(3):332-7.

Lim CY, Mat Junit S, Abdulla MA, Abdul Aziz A. In Vivo Biochemical and Gene Expression Analyses of the Antioxidant Activities and Hypocholesterolemic Properties of Tamarindus indica Fruit Pulp Extract. PLOS ONE. 2013;8(7):e70058.

Fagbemi KO, Aina DA, Adeoye-Isijola MO, Naidoo KK, Coopoosamy RM, Olajuyigbe OO. Bioactive compounds, antibacterial and antioxidant activities of methanol extract of Tamarindus indica Linn. Scientific Reports. 2022 ;12(1):9432.

Vasant RA, Narasimhacharya AVRL. Ameliorative effect of tamarind leaf on fluoride-induced metabolic alterations. Environ Health Prevent Med. 2012;17(6):484-93.




DOI: https://doi.org/10.18196/jfaps.v3i2.16708

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.


Journal of Fundamental and Applied Pharmaceutical Science are indexed by:

       

Office:

K.H. Sudja Building G3, 2nd Floor, Faculty of Medicine and Health Science, Universitas Muhammadiyah Yogyakarta,
Jalan Brawijaya (Lingkar Selatan), Tamantirto, Kasihan, Bantul, Daerah Istimewa Yogyakarta.
Whatsapp: +62 822-2155-6698
Email: jfaps@umy.university
Website: http://journal.umy.ac.id/index.php/jfap/index

Creative Commons License

Journal of Fundamental and Applied Pharmaceutical Science is licensed under a Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA 4.0) license.