Potential of Binahong Leaf Ethanol Extract (Anredera cordifolia (Ten.) Steenis) against Elevated HDL Levels (Rattus corvegious) Streptozonic-Induced Wistar Strain
Abstract
Diabetes mellitus is frequently associated with decreased levels of high-density lipoprotein (HDL), which are prevalent cardiovascular disease risk factors. The use of synthetic drugs substantially increases the risk of atherosclerosis since they often have severe side effects, require high costs, and have poor prescription adherence. Anredera cordifolia (Ten.) Steenis, often known as the binahong plant, is a potentially effective complementary therapy for increasing HDL levels. Flavonoid compounds present in the ethanol extract of Binahong leaf have the potential to increase HDL levels. This work aims to evaluate the action of an ethanol extract from binahong leaves in raising HDL levels in rats of the Streptozotocin-induced Wistar strain (Rattus norvegicus). In this research, the Pretest-Posttest Control Group Design was used. The study involved the grouping of five Wistar strain rats (n=5) into five distinct groups: one received binahong leaf ethanol extract at concentrations of 25 mg/kg BW, 50 mg/kg BW, and 100 mg/kg BW, which served as the positive control group and received a negative control group supplemented with atorvastatin at 0.9 mg/kg BW. The CHOD-PAP method was applied to measure HDL levels. SPSS software was used to run the normality test, Wilcoxon test, one-way ANOVA test, and paired sample t-test. No statistically significant difference in HDL levels was found between treatment groups. The 50 mg/kg BW binahong leaf extract group had significantly higher HDL levels (p-value < 0.05). As a result, the ethanol extract obtained from binahong leaves presumably raises HDL levels in streptozotocin-induced Wistar rats.
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Kemenkes RI. Hasil Riset Kesehatan Dasar Tahun 2018. Vol. 53 (9). 2018. p. 1689–1699.
Bhargava, S., de la Puente-Secades, S., Schurgers, L., & Jankowski J. Lipids and lipoproteins in cardiovascular diseases: a classification. Trends in Endocrinology and Metabolism. 2022;33 (6):409–23.
Srivastava RAK. Dysfunctional HDL in diabetes mellitus and its role in the pathogenesis of cardiovascular disease. Mol Cell Biochem. 2018;440 (1-2):167–187.
Chaudhury, D., & Aggarwal A. Diabetic Dyslipidemia: Current Conceptsin Pathophysiology and Management. J Clin Diagnostic Res. 2018;12 (1).
Rodriguez, F., Maron, D. J., Knowles, J. W., Virani, S. S., Lin, S., & Heidenreich PA. Association of Statin Adherence With Mortality in Patients With Atherosclerotic Cardiovascular Disease. JAMA Cardiol. 2019;4 (3):206–13.
Dwitiyanti, Harahap, Y., Elya, B., & Bahtiar A. Impact of solvent on the characteristics of standardized binahong leaf (Anredera cordifolia (Ten.) Steenis). Pharmacogn J. 2019;11 (6):1463–70.
Puspasari, A. F., Agustini, S. M., & Illahika AP. Pengaruh Ekstrak Daun Kersen (Muntingia calabra L.) terhadap Profil Lipid Mencit Putih (Mus musculus) Jantan yang Diinduksi Minyak Jelantah. Saintika Med. 2016;12 (1):49–55.
Pratiwi, W. R., Hediningsih, Y., & Isworo JT. Hubungan Kadar Glukosa Darah Dengan Kadar HDL (High Density Lipoprotein) Pada Pasien Diabetes Melitus Tipe 2. J Labora Med. 2021;5 (1):29–34.
Rampengan SH. MENINGKATKAN KOLESTEROL HDL Paradigma baru dalam pencegahan penyakit kardiovaskular. J Biomedik JBM. 2015;7 (2).
Warraich, H. J., & Rana JS. Dyslipidemia in diabetes mellitus and cardiovascular disease. Cardiovasc Endocrinol. 2017;6 (1):27.
Goyal, S. N., Reddy, N. M., Patil, K.R., Nakhate, K. T., Ojha, S. P, C. R., & Agrawal YO. Challenges and issues with streptozotocin-induced diabetes– a clinically relevant animal model to understand the diabetes pathogenesis and evaluate therapeutics. Chem Biol Interact. 2016;244:40–63.
Husna, F., Suyatna, F. D., Arozal, W., & Purwaningsih EH. Model hewan coba pada penelitian diabetes. Pharm Sci Res. 2019;6 (3):1.
Firdaus, Rimbawan, Anna Marliyati, S.,& Roosita K. Model Tikus Diabetes Yang Diinduksi Streptozotocin- Sukrosa Untuk Pendekatan Penelitian Diabetes Melitusgestasional Streptozotocin, Sucrose-Induce Diabetic Male Rats Model for Research Approach of Gestational Diabetes Mellitus. J Mkmi. 2016;12 (1):29–34.
Selawa, W., Runtuwene, M. R. J., & Citraningtyas G. Kandungan flavonoiddan kapasitas antioksidan total ekstrak etanol daun binahong [Anredera cordifolia(Ten.)Steenis. Pharmacon J Ilm Farm - UNSRAT. 2013;2 (1):18–23.
Sayuti M. Pengaruh perbedaan metode ekstraksi, bagian dan jenis pelarut terhadap rendemen dan aktifitas antioksidan bambu laut (Isis hippuris). Technol Sci Eng J. 2017;1 (3).
Mahmoud, M. F., El Ashry, F. E. Z. Z., El Maraghy, N. N., & Fahmy A. Studies on the antidiabetic activities of Momordica charantia fruit juice instreptozotocin-induced diabetic rats. Pharm Biol. 2017;55 (1):758–765.
Bahiru, E., Hsiao, R., Phillipson, D., & Watson KE. Mechanisms and treatment of dyslipidemia in diabetes. Curr Cardiol Rep. 2021;23:1–6.
Yunarto, N., Aini, N., Oktoberia, I. S., Sulistyowati, I., & Kurniatri AA. Aktivitas antioksidan serta penghambatan HMG CoA dan lipase dari kombinasi ekstrak daun binahong-rimpang temu lawak. J Kefarmasian Indones. 2019;89–96.
Cui, Y., Hou, P., Li, F., Liu, Q., Qin, S., Zhou, G., Xu, X., Si, Y., & Guo S. Quercetin improves macrophage reverse cholesterol transport in apolipoprotein E-deficient mice fed a high-fat diet. Lipids Health Dis. 2017;16:1–7.
Shargel L, Yu ABC. Applied biopharmaceutics and pharmacokinetics. 7th ed. McGraw-Hill Education; 2016.
März, W., Kleber, M. E., Scharnagl, H., Speer, T., Zewinger, S., Ritsch, A., Parhofer, K. G., von Eckardstein, A., Landmesser, U., & Laufs, U.März, W., Kleber, M. E., Scharnagl, H., Speer, T., Zewinger, S., Ritsch, A., Parhofer, K. G., von Eckardstein, A. U. HDL cholesterol: reappraisal of its clinical relevance. Clin Res Cardiol. 2017;106:663–675.
Tangvarasittichai S. Oxidative stress, insulin resistance, dyslipidemia and type 2 diabetes mellitus. World J Diabetes. 2015;6 (3):456.
DOI: https://doi.org/10.18196/jfaps.v5i1.22496
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