Motilitas dan Viabilitas Spermatozoa Mencit ( Mus musculus) Setelah Pemberian Solasodin yang Diisolasi dari Terong Kuning (Solatium khasianum)

Alfaina Wahyuni

Abstract


The eggplant is consumed and used in traditional medication, for example to reduce male desire. One of the solanum’s alkaloid which may affect on reproduction is solasodine , but it is still unclear. This study was conducted to investigate the effects of solasodine on sperm motility and viability in adult mice.
Twenty five healthy mice, 3 months old, and 37- 45 gram of body weight were used. They were divided into five groups. Each group consisted of five mice. Group I, control without any treatment (Kl), group II, treatment with solvent of solasodine, polyvinylpirollidon 1% in aquadest (K2), group III, treatment with solasodine 87,61 mg/kgBw/day (PI), group IV, treatment with solasodine 175,62 mg/kgBW/day (P2) and group V, treatment with solasodine 263,43 mg/kgBW/day (P3). Treatments were given in 36 days. In the 37th day mice were killed for evaluation of sperm motility and viability. The result of this study shows that sperm motility and viability in all treatment groups were significantly reduced (Analysis of Variance cintinued with least significant difference, p < 0,05).

Terong banyak dikonsumsi masyarakat dan digunakan sebagai bahan obat osional misalnya untuk menurunkan nafsu seks pria. Salah satu alkaloid solanum ;;~‘Z kemungkinan berpengaruh terhadap fungsi reproduksi adalah solasodin, tetapi . mi masih belum jelas dan perlu penelitian lebih lanjut, Penelitian ini dilakukan eik mengetahui pengaruh pemberian solasodin terhadap kualitas spermatozoa lisusnya motilitas dan viabilitas spermatozoa mencit dewasa.
Digunakan 25 ekor mencit jantan umur 3 bulan, sehat, berat 37-45 gram.Mencit i fiompokkan menjadi 5 kelompok, tiap kelompok terdiri dari 5 ekor mencit. i rmpok I, kontrol tanpa perlakuan apapun (Kl), kelompok II, diberi larutan ' vinylpyrollidon 1% dalam aquadest(K2), kelompok III, diberi perlakuan solasodin ;: s 87,81 mg/kgBB/hr (Pl), kelompok IV, diberi solasodin dosis 175,63 mg/kgBB/ in kelompok V diberi solasodin 263,43 mg/kgBB/hr. Perlakuan diberikan selama : izii kemudian pada hari ke-37 semua mencit dimatikan dan diambil cauda :ijd\misnya untuk pemeriksaan motilitas dan viabilitas spermatozoa. Hasil penelitian lezunjukkan bahwa pada semua kelompok perlakuan terjadi penurunan motilitas BE iabilitas spermatozoa secara bermakna (Analisis varian dilanjutkan dengan uji 12 nyata terkecil, p < 0,05).

Keywords


solasodin; motilitas; viabilitas spermatozoa; solasodine; sperm- motility; viability

Full Text:

PDF

References


Asmarindali dan O. Soeradi, 1994, Pengaruh Ekstrak Biji Pepaya In Vitro terhadap Kualitas Sperma¬tozoa manusia. Maj. Kedokteran Indonesia, vol. 114,No. 10, Oktober, 1994.

Arsyad, KLM, 1986. MacamKontrasepsi Pria Masa Depan, dalam : Peranan Pria dalam Keluarga Berencana, Prosiding Pertemuan Ilmiah Tahunan PANDI, Penerbit tarsito, Bandung.

Bardin,C.W.,Cheng,C.Y.,Musto,N.E., and Gunsalus,G.L. 1988. The Sertoli Cell. Dalam: Knobil.E, and Neil,J. (eds). The Phisiology of Reproduction. Raven press,Ltd., New York.

Bedford, J.M. 1975. Maturation, Transport and Fate of Spermatozoa in the Epididymis. Dalam: Greep, R.O. Astwood E.B., Hamilton, D.W. and Geiger, S.R. (eds). Handbook of Physiology, Section Endocrinology vol. V. Waverly Press, Inc., Baltimore.

Claringbold,W.D.B., Few,J.D., Brace, C J., and Renwick,J.H. 1980. The Disposition and Metabolism of (3H) Solasodin in Man and Hamster. Journ.SteroidBiochem. 13: 889-93

Cornwall,G.A., Smyth,T.B., Vindirich,D. Harter,C., Robinson,J and Chang,T.S.K. 1986. Induction and Enhancement of Progressive Motility in Hamster Caput Epididymal Spermatozoa. Biol. Reprod. (35): 1065 - 74.

Danzo,B.J. 1995. The Effect of Gonadotropin-Releasing Hormone Antagonist on ABP Distribution and Other Parameter in The Adult Male Rat. Endocrinology. 13(9): 4004 - 11.

Dixit, V.P., Gupta,R.S., and Gupta, S.1989. Antifertility Plant Products: Testiculair Cell Populations Dynamic Following Solasodin (C27H4302N) Administration in Rhesus Monkey (Macaca malata) Journ. Andrologia : 21 (6): 542-6.

Fasich. 1981. Isolasi Diosgenin dan Solasodin serta Studi Antifertilitas Solasodin dan Hasil Oksidasinya. Disertasi Doktor. Institut Teknologi Bandung.

First,N.I.1971. Collection and Preservation of Spermatozoa. Dalam: Joseph,C and Daniel,Jr.(eds) Methode in Mammalian Embryology . Toppan Company Limited. Japan.

Ghufron,M dan Herwiyanti,S. 1995. Gambaran Histologik Spermatogenesis Tikus Putih (Ratus norvegicus) Setelah diberi Makan Terong Tukak (Solanum torvum). Jurnal Kedok YARSI. 3(2): 28-35

Hadley, M.E. 1992. Endocrinology. Prentice Hall Intema-tional, London.

Hakim,AdanTatang,S. 1980. Inventarisasi Tanaman Solanacea Indonesia sebagai Sumber Solasodin. Risalah Simposium Penelitian Tumbuhan dan Obat II. Departemen Fisiologi dan Farmakologi FKH. IPB.Bogor

Istriyati. 1983. Pengaruh Solasodin terhadap Perkembangan Saluran Telur Puyuh (Coturnix coturnix japonica) muda. Tesis Fakultas Pasca Sarjana. Institut Teknologi Bandung.

runquera,L.C.,Carneiro,J., and Kelley,R.O. 1995. Basic Histology.8th. ed. Prentice- Hall International,London

Martin -du Pan,R. and Campana.1993. Phisiopathology of Spermatogenic Arrest. Fertil. Steril. 60 (6):937-51

Noris,D.O.,1980, Vertebrate Endocrinology, LeaandFebige Co., Pilladelphia.

Ihgebin-Crist, M.C., Danzo, B.J. and Davies, J. 1975. Endocrine Control of The Development and Maintenance of Sperm Fertilizing Ability in The Epididymis. Dalam: Greep, R.O. Astwood E.B., Hamilton, D.W. and Geiger, S.R. (eds). Handbook of Physiology, Section Endocrinology vol. V. Waverly Press, Inc., Baltimore.

Rdbaire, B and Viger, R.S., 1995. Regulation of Epididymal Epithelial Cell Functions. Biol. Reprod. 52 (2): 226-36.

ugh,R. 1968. The Mouse. Its Reproduction and Development. Burgers Publishing Co. Mineapolis.

SchenckjB. and Neumann,F. 1978. Influence of Antiandrogen on Sertoli Cell Function and Intra Testiculair Androgen Transport. Int. J. Androl. (1):459 - 469.

Soenarjo, Ch. 1986. Pengendalian Nafsu Seks Suami Dalam Mengatur Kehamilan Istri dan Kelahiran Anak. Konas VIPANDI: Yogyakarta.

jgiyanto,Y.1983. Efek Solasodin Terhadap Perkembangan Embrio Mencit Putih (Mus musculus) Galur Australia. Tesis. Fakultas pasca Sarjana. Institut Teknologi Bandung.

TzrigamP. 1980. Beberapa Aspek Kimia Sapogenin Steroid Pada tumbuhan di Indonesia. Penerbit Alumni. Bandung

Yoglmayr,J.K. 1975. Metabolic Change in Spermatozoa During Epididymal Transit. Dalam: Greep, R.O. Astwood E.B., Hamilton, D.W. and Geiger, S.R. (eds). Handbook of Physiology, Section Endocrinology vol. V. Waverly Press, Inc., Baltimore.

WHO. 1992.Penuntun Laboratorium WHO untuk Pemeriksaan Semen Manusia dan Interaksi Sperma- Getah Servik. ed. 3. Bagian Biologi Medik. Fakultas Kedokteran. Universitas Sriwijaya.

U :;ayakusuma,H.M., Wirian,A.S., Yaputra,S., Dalimartha,S., dan Wibowo,B. 1994. Tanaman Berkhasiat Obat di Indonesia. Jilid ke-2. Pustaka Kartini. Jakarta.

Williams - Ashman,H.G.1988. Perspective in Male Reproduction. In: E. Knobil dan J.D.Neil (eds). The Physiology of Reproduction. Raven Press. NewYork




DOI: https://doi.org/10.18196/mmjkk.v1i2.1899

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.


      

Editorial Office:
Journal Room, G1 (Biomedic) Building, Ground Floor, Faculty of Medicine and Health Science Universitas Muhammadiyah Yogyakarta, 
Jalan Lingkar Selatan (Brawijaya), Tamantirto, Kasihan, Bantul, Daerah Istimewa Yogyakarta, Indonesia
Phone: +62 274 387 656 (ext: 231)
WA : +62 811-2650-303
Website: http://journal.umy.ac.id/index.php/mm 
E-mail: mmjkk@umy.university

Creative Commons License
Mutiara Medika: Jurnal Kedokteran dan Kesehatan is licensed under a Creative Commons Attribution 4.0 International License. View My Stats