Effects of AntiMycobacterium Tuberculosis Nanoherbal Legundy Leaf Extract (Vitex trifolia)

Atika Dwiyanti, Siti Mulyani, Oche Axena Yulhan, Humairah Medina Liza Lubis, Ance Roslina

Abstract


Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. Indonesia ranks 2nd largest in the world after India. Resistance to anti-tuberculosis drugs is one of the difficulties in treating tuberculosis. This research aim to determine the effectiveness of legundi (Vitex trifolia) leaf extract nanoherbal in killing Mycobacterium tuberculosis. This study was conducted with an experimental design using M. tuberculosis colonies taken from 25 samples of aspirate of tuberculosis patients with lymph nodes and then given the ethanol extract of legundi leaves (70% and 90%) with the process of making nanoparticle extracts. The evaluation of the observations was in the form of measuring the inhibition zone for bacterial growth which were categorized as weak (<5mm), moderate (6-10mm), strong (11-20 mm) and very strong (> 21mm). The results of the study from 6 repetitions found that the inhibition zone category was 0% weak, 39% moderate, strong 52.3%, and very strong 8.7%. Comparison of the concentration of legundi leaf extract to the diameter of the bacterial growth inhibitory zone was analyzed using the Kruskal Wallis test, which found a significant relationship with p value = 0.000 (<0.05). Legundi's leaf extract can be used as an antibacterial agent for M. tuberculosis.

 


Keywords


Vitex trifolia leaf extract; antibacterial; Mycobacterium tuberculosis

Full Text:

PDF

References


Banuls AL, Sonou A, Van Anh NT, Godreuil S. Mycobacterium tuberculosis : ecology and evolution of a human bacterium. J Med Microbiol. 2015 Nov; 64 (11): p. 1261-1269.

Dinas Kesehatan Provinsi Sumatera Utara. Profil Kesehatan Kota Medan. Medan: 2016 [cited 2020 Mar 10]. Available from: Profil Kes Prov Sumut 2016 eBooks.

Yunita SI, Kusmiati T. TB MDR Primer dengan Limfadenitis TB pada Wanita SLE. J Respirasi: 2015 Sep; 1 (3): p. 81-87.

Kemenkes RI. Profil Kesehatan Indonesia 2017. Indonesia : 2018. [cited 2020 Mar 10]

Flynn JL, Chan J. Immunology of Tuberculosis. Annual Review of Immunology. 2001 Apr;19 (1); p. 93–129.

Perhimpunan Dokter Paru Indonesia. Tuberkulosis Pedoman Diagnosis dan Penatalaksanaan di Indonesia. Jakarta: [cited 2020 Mar 20] PDPI; 2006.

Mustanir dan Rosnani. Isolasi Senyawa Bioaktif Penolak (Repellent) Nyamuk dari Ekstrak Aseton Batang Tumbuhan Legundi (Vitex trifolia). Buletin Littro. 2008 ;19 (2): p. 174-180

Listiawati Y. Uji Efek Antipiretik Ekstrak Etanol Daun Legundi (Vitex trifolia Linn) pada Kelinci yang Diinduksi Vaksin Dpt-Hb. Universitas Muhammadiyah Surakarta. 2010 [cited 2011 Jan 31]. Available from : http://eprints.ums.ac.id/10093

Tiwari N, Thakur J, Saikia D, Gupta MM. Antitubercular diterpenoids from Vitex trifolia. Phytomedicine. 2013 May 15;20(7):605-10.

Lubis HML, Hariaji I. Ekstrak Buah Legundi (Vitex trifolia) Mampu Menghambat Pembelahan dan Pertumbuhan Sel Tumor Kulit Tikus. Mutiara Medika Jurnal Kedokteran dan Kesehatan. 2017 Jan 17 (1): p. 1-6.

Jawetz, Melnick, Adelberg. Mikrobiologi Kedokteran. 27th ed. Jakarta: EGC; 2016

Anggraini A, Yuniningsih S, Sota MM. Pengaruh Ph Terhadap Kualitas Produk Etanol dari Molasses melalui Proses Fermentasi. Jurnal Reka Buana. 2017; 2 (2): p. 99-105.

Mahardika HA, Sarwiyono, Surjowardojo P. Ekstrak Metanol Daun Kersen (Muntingia calabura L) sebagai Antimikroba Alami terhadap Bakteri Staphylococcus aureus Penyebab Mastitis Subklinis pada Sapi Perah. Jurnal Ternak Tropika. 2014;15 (2): p. 15-22.

Geetha V, Doss A, Doss AP. Antimicrobial Potential of Vitex trifolia Linn. Anc Sci Life. 2004 Apr; 23 (4): 30-2

Amanda EA, Oktiani BW, Panjaitan FUA. Efektivitas Antibakteri Ekstrak Flavonoid Propolis Trigona Sp (Trigona thorasica) terhadap Pertumbuhan Bakteri Porphyromonas gingivalis. Jurnal Kedokteran Gigi. 2019; 3 (1): p. 23-28.




DOI: https://doi.org/10.18196/mmjkk.v21i1.6497

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

      

Editorial Office:
Journal Room, G1 (Biomedic) Building, Ground Floor, Faculty of Medicine and Health Science Universitas Muhammadiyah Yogyakarta, 
Jalan Lingkar Selatan (Brawijaya), Tamantirto, Kasihan, Bantul, Daerah Istimewa Yogyakarta, Indonesia
Phone: +62 274 387 656 (ext: 231)
WA : +62 811-2650-303
Website: http://journal.umy.ac.id/index.php/mm 
E-mail: mmjkk@umy.university

Creative Commons License
Mutiara Medika: Jurnal Kedokteran dan Kesehatan is licensed under a Creative Commons Attribution 4.0 International License. View My Stats