Pengaruh Durasi Pemberian Ekstrak Etanol Pegagan (Centella asiatica sp.) terhadap Memori Spasial Tikus Putih (Rattus norvegicus) pascastres Listrik
DOI:
https://doi.org/10.18196/mmjkk.v11i2.932Keywords:
Centella asiatica, stres, memori spasial, maze radialAbstract
Beberapa penelitian telah dilakukan berkaitan dengan kemampuan pegagan sebagai neurotropik dan neuroprotektif. Tujuan penelitian ini untuk mengungkapkan pengaruh durasi pemberian ekstrak etanol pegagan dalam peningkatan memori spasial tikus putih pascastres. Pada penelitian ini, 21 tikus jantan, usia delapan minggu dibagi dalam tiga kelompok: dua kelompok perlakuan (K1 dan K2) dan satu kelompok kontrol (KN). Kelompok perlakuan menerima ekstrak ethanol pegagan sebesar 150 mg/kgBB/ ml secara oral selama empat(K1) dan enam(K2) minggu. Kelompok kontrol akan menerima aquades 1 ml selama enam minggu. Semua kelompok akan diuji memori dengan menggunakan maze radial delapan lengan selama 12 hari sebelum dan setelah perlakuan. Uji stres listrik selama 10 menit dilakukan sebelum perlakuan. Hasil penelitian menunjukkan persentase tertinggi ketepatan pemilihan lengan dalam uji maze radial 2 (UMR2) untuk KN, K1 dan K2 masing-masing sebesar 23,6%, 44,8% dan 91,71%, dengan rerata persentase masing-masing sebesar 10,24%, 14,12% dan 53,33%. Uji Mann-Whitney menunjukkan bahwa persentase ketepatan pemilihan lengan berbeda secara bermakna antara satu kelompok dengan kelompok lainnya. Kesimpulannya adalah pegagan mampu meningkatkan memori spasial tikus putih pascastres dan pemberian ekstrak etanol pegagan selama enam minggu memberikan efek peningkatan memori yang lebih signifikan dibanding pemberian selama empat minggu.
References
Syamsuhidayat, S.S., Hutapea, J.R. 1991. Inventaris Tanaman Obat Indonesia. Jakarta: Departemen Kesehatan RI.
Ramasamy I. 2005. AgriInfoTech, Inc. 166 Lawrence Road, salem NH-USA 03079 Ph:603-894-7346, 603-781-9097.Available at www.agriinfotech.com.
Anon. Centella asiatica. Bangalore, India: Natural Remedies Pvt.Ltd. 1997.
Mas’ud, I. 2003. Stres Fungsional Dapat Menjadi Salah Satu Pemicu Hilangnya Memori? Thesis. Fakultas Kedokteran Universitas Brawijaya. Malang.
Lupien, S.J., Gaudreau, S., Tchiteya, B.M., Maheu, F., Sharma, S., Nair, N.P.V., et al. 1997. Stres-induced declarative memory impairment in healthy elderly subjects: relationship to cortisol reactivity. J. Clin. Endocrinol. Metab. 82(7):2070-2075.
Simonian, N.A., Coyle, J.T. 1996. Oxidative Stress In Neurodegenerative Disorders. Ann. Review. Pharmacol. Toxicol. 36:83-106
McEwen, B.S., Sapolsky, R.M. 1995. Stress and cognitive function. Curr. Opin. Neurobiol. 5: 205-16.
Kerr, D.S., Campbell, L.W., Thibault, O., Landfield, P.W. 1992. Hippocampal glucocorticoid receptor activation enhances voltage-dependent Ca2+ conductances: relevance to brain aging. Proc. Natl. Acad. Sci. USA. 89:852731.
Scoville, W.B., Milner, B. 1957. Loss of Recent Memory after Bilateral Hippocampal Lesions. J. Neurol. Neurosurg. Psych. 20:11–21.
Poirier, G.L., Amin, E., John, P. 2008. Qualitatively Different Hippocampal Subfield Engagement Emerges with Mastery of a Spatial Memory Task by Rats. The Journal of Neuroscience. 28(5):1034 –1045.
Wong, P.T. 2007. Hippocampal long-term depression mediates acute stress-induced spatial memory retrieval impairment. PNAS 104(27): 11471–11476.
Fadda, F., Melis, M.R., Argiolas, A. 1978. Effect of electric foot shock on dopamine and 3,4-dihydroxyphenylacetic acid (DOPAC) in different brain areas of rats. Boll. Soc. Ital. Biol. Sper. 1978;54(18):1747-50.
Tissari, A.H., Argiolas, A., Fadda, F., Serra, G., Gessa, G.L. 1979. Foot-Shock Stress Accelerates Non-Striatal Dopamine Synthesis Without Activating Tyrosine Hydroxylase. NaunynSchmiedeberg’s Arch. Pharmacol. 308:155157.
Pani, L., Porcella, A., Gessa, G.L. 2000. The role of stress in the pathophysiology of the dopaminergic system. Molecular Psychiatry. 5:14-21.
Anon. Farmakope Indonesia. Jakarta: Departermen Kesehatan Republik Indonesia. 1979.
Soumyanath, A., Zhong, Y.P., Gold, S.A., Yu, X., Koop, D.R., Bourdette, D., Gold, B.G. 2005. Centella asiatica accelerates nerve regeneration upon oral administration and contains multiple active fractions increasing neurite elongation in-vitro. Journal of Pharmacy and Pharmacology. 57(9):1221-29.
Rao, K.G.M., Rao, S.M., Rao, S.G. 2006. Centella asiatica (L.) Leaf Extract Treatment During the Growth Spurt Period Enhances Hippocampal CA3 Neuronal Dendritic Arborization in Rats. eCAM 3(3):349–357.
Rao, K.G.M., Rao, S.M., Rao, S.G. 2007. Enhancement of amygdaloid neuronal dendritik arborization by fresh leaf juice of Centella asiatica (Linn) during growth spurt period in rats. eCAM: 1-8.
Bradwejn, J., Zhou, Y., Koszycki, D., Shlik, J. 2000 . A double-blind, placebo-controlled study on the effects of Gotu Kola (Centella asiatica) on acoustic startle response in healthy subject. J. Clin. Psychopharmacol. 20(6): 680-4.
Rao, K.G.M., Rao, S.M., Rao, S.G. 2005. Centella asiatica (linn) induced behavioral changes during growth spurt period in neonatal rats. Neuroanatomy:4:18-23.
Nalini, K., Aroor, A.R., Karanth, K.S., Rao, A. 1992 . Effect of Centella asiatica fresh leaf aqueous extract on learning and memory and biogenic amine turnover in albino rats. Fitoterapia; 63:232–8.
Mook-Jung, I., Shin, J.E., Yun, S.H., Huh, K., Koh, J.Y., Park, H.K., et al. 1999 . Protective effects of asiaticoside derivates against betaamyloid neurotoxicity. J. Neurosci. Res. 59(3): 417-25.
Kumar, M.H.V., Gupta, Y.K. 2002. Effect of different extracts of Centella asiatica on cognition and markers of oxidative stress in rats. 79(2): 253-260.
Berman, A.F. 2003. The 5-minute Herb and Dietary Supplement Consult. Philadelphia: Lippincott Williams & Wilkins.
Downloads
Published
Issue
Section
License
Copyright
Authors retain copyright and grant Mutiara Medika: Jurnal Kedokteran dan Kesehatan (MMJKK) the right of first publication with the work simultaneously licensed under an Attribution 4.0 International (CC BY 4.0) that allows others to remix, adapt and build upon the work with an acknowledgment of the work's authorship and of the initial publication in Mutiara Medika: Jurnal Kedokteran dan Kesehatan (MMJKK).
Authors are permitted to copy and redistribute the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Mutiara Medika: Jurnal Kedokteran dan Kesehatan (MMJKK).
License
Articles published in the Mutiara Medika: Jurnal Kedokteran dan Kesehatan (MMJKK) are licensed under an Attribution 4.0 International (CC BY 4.0) license. You are free to:
- Share — copy and redistribute the material in any medium or format.
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
This license is acceptable for Free Cultural Works. The licensor cannot revoke these freedoms as long as you follow the license terms. Under the following terms:
Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
