Evaluation of the Antithrombotic Activity of Acmella oleracea L. Flower Ethanol Extract

Amanda Gita Tiaravista, Laras Widawaty Putri, Arief Nurrochmad, Sitarina Widyarini, Nanang Fakhrudin

Abstract


Acmella oleracea L. (hereinafter abbreviated as AOE) is one of the plants with the potential for antithrombosis, one of the deadliest cardiovascular diseases in Indonesia. The antithrombotic activity test on AOE leaf extract revealed that it could lyse blood clots. However, no information regarding the AOE flower as an antithrombosis is provided. This study, thus, aims to determine the chromatography profile and the antithrombotic activity of the AOE flower ethanol extract. To identify the AOE chemical profile, thin-layer chromatography was carried out. Antithrombotic testing was performed on male rats of the Sprague-Dawley strain. Then, the antithrombotic activity was tested using the FeCl3-induced rat method, with the observed parameter being total occlusion time. The test animals were also divided into six groups: normal, solvent (CMC-Na 0.9%), comparator drug (clopidogrel 8.67 mg/kg), and AOE (doses 125, 250, and 375 mg/kg). The data obtained were then analyzed statistically using Kruskal-Wallis, followed by Tukey's. The TLC profile results confirmed the presence of the alkaloid compound in AOE. The authors also found that AOE at doses of 125, 250, and 375 mg/kg significantly prolonged the occlusion time comparable to that of clopidogrel at 8.67 mg/kg (p > 0.05). This finding indicates that AOE has antithrombotic activity in FeCl3-induced rats.

Keywords


Acmella; alkaloid; cardiovascular diseases; spilanthol; thrombosis; toothache plant

Full Text:

PDF

References


WHO. Cardiovascular diseases (CVDs) [Internet]. Cardiovascular diseases (CVDs). 2021. Available from: https://www.who.int/news-room/fact-sheets/detail/cardiovascular-diseases-(cvds).

Barrett KE, Barman SM, Boitano S, Brooks HL. Blood as a Circulatory Fluid & the Dynamics of Blood & Lymph Flow. 24th ed. New York: McGraw-Hill Education LLC; 2019; (Ganong’s Review of Medical Physiology).

Kim K, Do HJ, Oh TW, Kim KY, Kim TH, Ma JY, et al. Antiplatelet and antithrombotic activity of a traditional medicine, hwangryunhaedok-tang. Front Pharmacol. 2019;9. http://dx.doi.org/10.3389/fphar.2018.01502

Arsyad AS, Nurrochmad A, Fakhrudin N. Phytochemistry, traditional uses, and pharmacological activities of Ficus elastica Roxb. ex Hornem: A review. J Herbmed Pharmacol. 2022;12(1):41–53. http://dx.doi.org/10.34172/jhp.2023.04

Putra IMWA, Fakhrudin N, Nurrochmad A, Wahyuono S. A review of medicinal plants with renoprotective activity in diabetic nephropathy animal models. Life (Basel). 2023;13(2):560. http://dx.doi.org/10.3390/life13020560

Maha Dewi NKS, Fakhrudin N, Wahyuono S. A comprehensive review on the phytoconstituents and biological activities of Nyctanthes arbor-tristis L. J Appl Pharm Sci. 2022;9–17. http://dx.doi.org/10.7324/japs.2022.120802

Nurhidayati LG, Nugroho AE, Retnoaji B, Sudarsono S, Fakhrudin N. Antiangiogenesis Activity Study of Awar-Awar Leaf Ethanolic Extract (Ficus Septica Burm. F.) By Chick Chorioallantoic Membrane Method. Indones J Pharm. 2021 http://dx.doi.org/10.22146/ijp.607

Hastuti I, Nurrochmad A, Puspitasari I, Fakhrudin N. Studi Aktivitas Antiplatelet dan Antitrombosis Ekstrak Air Daun Sukun (Artocarpus altilis (Park.) Fosberg). J Tumbuh Obat Indones. 2021;14(1):85–94. http://dx.doi.org/10.22435/jtoi.v14i1.4227

Uthpala TGG, Navaratne SB. Acmella oleracea plant; Identification, applications and use as an emerging food source – review. Food Rev Int. 2021;37(4):399–414. http://dx.doi.org/10.1080/87559129.2019.1709201

Rondanelli M, Fossari F, Vecchio V, Braschi V, Riva A, Allegrini P, et al. Acmella oleracea for pain management. Fitoterapia. 2020;140(104419):104419. http://dx.doi.org/10.1016/j.fitote.2019.104419

Tabassum F, Chadni SH, Mou KN, Hasif I, Ahmed T, Akter M. In-vitro thrombolytic activity and phytochemical evaluation of leaf extracts of four medicinal plants of Asteraceae family. 2017;6(4):1166-1169.

Maimulyanti A, Prihadi AR, Safrudin I. Chemical composition, phytochemical screening and antioxidant activity of Acmella uliginosa (SW.) Cass leaves. Indones J Chem. 2018;16(2):162. http://dx.doi.org/10.22146/ijc.21160

Leopold JA. Antioxidants and coronary artery disease: From pathophysiology to preventive therapy. Coron Artery Dis. 2015;26(2):176–83. http://dx.doi.org/10.1097/mca.0000000000000187

Li W, Nieman M, Sen Gupta A. Ferric chloride-induced Murine thrombosis models. J Vis Exp. 2016;(115). http://dx.doi.org/10.3791/54479

Fakhrudin N, Franyoto YD, Astuti ED, Nurrochmad A, Wahyuono S. The effect of ursolic acid from Plantago lanceolata leaves on leukocytes migration and chemokines level. Indones J Pharm. 2019;30(4):252. http://dx.doi.org/10.14499/indonesianjpharm30iss4pp252

Rijai HR, Fakhrudin N, Wahyuono S. Isolation and Identification of DPPH Radical (2,2-diphenyl-1-pikrylhidrazyl) Scavenging Active Compound in Ethyl acetat fraction of Piper acre Blume. Maj Obat Tradis. 2019;24(3):204. http://dx.doi.org/10.22146/mot.48173

Dias AMA, Santos P, Seabra IJ, Júnior RNC, Braga MEM, de Sousa HC. Spilanthol from Spilanthes acmella flowers, leaves and stems obtained by selective supercritical carbon dioxide extraction. J Supercrit Fluids. 2012;61:62–70. http://dx.doi.org/10.1016/j.supflu.2011.09.020

Yumni GG. Uji Aktivitas Antitrombosis isolate 2-Geranil-2’,3,4,4’ Tetrahidroksi Dihidrokalkon dari Daun Sukun (Artocarpus altilis (Park.) Fosnerg). Tesis [Yogyakarta]: Universitas Gadjah Mada; 2021.

Wang X, Smith PL, Hsu MY, Ogletree ML, Schumacher WA. Murine model of ferric chloride‐induced vena cava thrombosis: evidence for effect of potato carboxypeptidase inhibitor. J Thromb Haemost. 2006;4(2):403–10. http://dx.doi.org/10.1111/j.1538-7836.2006.01703.x

Wang X, Xu L. An optimized murine model of ferric chloride-induced arterial thrombosis for thrombosis research. Thromb Res. 2005;115(1–2):95–100. http://dx.doi.org/10.1016/j.thromres.2004.07.009

Lalsangpuii F, Rokhum SL, Nghakliana F, Fakawmi L, Ruatpuia JVL, Laltlanmawii E, et al. Green synthesis of silver nanoparticles using Spilanthes acmella leaf extract and its antioxidant-mediated ameliorative activity against doxorubicin-induced toxicity in Dalton’s lymphoma ascites (DLA)-bearing mice. ACS Omega. 2022;7(48):44346–59. http://dx.doi.org/10.1021/acsomega.2c05970

Memariani Z, Moeini R, Hamedi SS, Gorji N, Mozaffarpur SA. Medicinal plants with antithrombotic property in Persian medicine: a mechanistic review. J Thromb Thrombolysis. 2018;45(1):158–79. http://dx.doi.org/10.1007/s11239-017-1580-3

Ahmad N, Lesa KN, Sudarmanto A, Fakhrudin N, Ikawati Z. The role of Phosphodiesterase-1 and its natural product inhibitors in Alzheimer’s disease: A review. Front Pharmacol. 2022;13. http://dx.doi.org/10.3389/fphar.2022.1070677

Nurhasanah D, Fakhrudin N, Retnoaji B, Nugroho A. Antiangiogenic activity of n-hexane insoluble fraction and its tylophorine component from ficus septica leaves in chicken chorioallantoic membrane induced by bFGF. Asian Pac J Cancer Prev. 2023;24(1):75–80. http://dx.doi.org/10.31557/apjcp.2023.24.1.75

Pramiastuti O, Wahyuono S, Fakhrudin N, Astuti P. Phytochemical and Pharmacological Activities of Curcuma purpurascens Blume, A Review. J Trop Biodivers Biotechnol. 2023;8(1):75891. http://dx.doi.org/10.22146/jtbb.75891

Triastuti A, Pradana D, Setiawan I, Fakhrudin N, Himmi S, Widyarini S, et al. In vivo anti-inflammatory activities of Plantago major extract and fractions and analysis of their phytochemical components using a high-resolution mass spectrometry. Res Pharm Sci. 2022;17(6):665. http://dx.doi.org/10.4103/1735-5362.359433




DOI: https://doi.org/10.18196/jfaps.v4i1.19055

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Journal of Fundamental and Applied Pharmaceutical Science are indexed by:

       

Office:

K.H. Sudja Building G3, 2nd Floor, Faculty of Medicine and Health Science, Universitas Muhammadiyah Yogyakarta,
Jalan Brawijaya (Lingkar Selatan), Tamantirto, Kasihan, Bantul, Daerah Istimewa Yogyakarta.
Whatsapp: +62 822-2155-6698
Email: jfaps@umy.university
Website: http://journal.umy.ac.id/index.php/jfap/index

Creative Commons License

Journal of Fundamental and Applied Pharmaceutical Science is licensed under a Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA 4.0) license.