Pencapaian Fase Embriosomatik Manggis (Garcinia mangostana L.) dengan Penambahan Thidiazuron dalam Medium Setengah MS Cair

Innaka Ageng Rineksane


One of the problems related to the establishment of mangosteen plantation is to obtain seedlings throughout the year, which can be solved by micropropagation.   The propagation of Mangosteen was done through somatic embryogenesis. The objective of this study was to determine the effect of Thidiazuron concentration in ½ MS medium to achieve somatic embryo stages of mangosteen seed. The study consisted of two experiments. Experiment 1: The embryogenic callus c.a.1 g derived from MS solid medium containing of Thidiazuron (0,1; 0,5; dan 1 mg/l) and 2,4-Dichlorophenoxy acetic acid (4,6,8 dan 10 mg/l) was subcultured into ½ MS0 liquid medium. 2). The embryogenic callus from the previous medium were subcultured into ½ MS liquid medium containing Thidiazuron (0, 1, 2, 4 dan 8 mg/L) and Casein hydrolysate 500 mg/L. The results showed that cell suspension were developed after mangosteen embryogenic callus subcultured into ½ MS0 liquid medium.  Somatic embryo stages such as globular, heart and torpedo were formed after the calli derived from the previous ½ MS solid medium containing 10 mg/L 2,4-D and 1 mg/L Thidiazuron were subcultured into ½ MS0 liquid medium in 8 weeks.  Cell suspension in ½ MS liquid medium with or without the addition of  Thidiazuron and Casein hydrolysate has induced the formation of embryo somatic stages such as globular, heart and torpedo after 6 months of incubation.


Mangosteen; Somatic Embryo Stage; Thidiazuron; ½ MS Liquid Medium

Full Text:



Dodds, J.H., and L.W. Roberts. 1982. Experiments in Plant Tissue Culture Cambridge University Press, Cambridge. 178p.

Gamborg, O.L., and G.C. Phillips. 1995. Plant Cell, Tissue and Organ Culture Fundamental Methods Springer-Verlag, Berlin Heidelberg. 358p

Osman, M. and A.R. Milan. 2006. Mangosteen-Garcinia mangostana. Southampton Centre for Underutilised Crops. University of Southampton.Southampton, UK. 170p.

Pedraza-Chaverri, J., N. Cardenas-Rodriguez, M. Orozco-Ibarra and J.M. Perez-Rojas.2008. Medicinal properties of mangosteen (Garcinia mangostana). Food and Chemical Toxicology 46:3227 – 3239.

Razdan, M.K. 2005.Introduction to Plant Tissue Culture.2nd ed. Science Publishers, Inc, New Hampshire. 375p.

Rineksane, I.A. 2011. Embriogenesis, Organogenesis and Assessment of Somaclonal Variations in Mangosteen (Garcinia mangostana L.).PhD Thesis. Universiti Putra Malaysia

Rineksane,I.A., M.A. Kadir, S. Kadzimin and F.Q. Zamaruzaman 2012. In vitrodevelopment of embryogenic calli and embryogenic stages in suspension cultures of mangosteen (Garcinia mangostana L.). Journal of Medical Plants Reseach 6 (13):2549 – 2559.

Rineksane, I.A., W. Aprillyastuti dan Agung Astuti. 2013. Pengaruh Jenis Eksplan, Thidiazuron dan 2,4-D terhadap Induksi Kalus Embriogenik Manggis (Garcinia mangostana L.) pada Medium Setengah MS. Prosiding Seminar Ilmiah Perhorti 2013, Vol.1. ISBN 978-979-25-1267-0.Perhimpunan Hortikultura Indonesia.

Suksamrarn, S., O. Komutiban, P. Ratananukul, N. Chimnoi, N. Lartpornmatulee and A. Suksamrarn. 2006. Cytotoxic prenylated xanthones from the young fruit of Garcinia mangostana. Chemical Pharmacy Bulletin 54:301-305.

Te-chato S, M. Lim and P. Suranilpong. 1995. Embryogenic callus induction in mangosteen (Garcinia mangostana L). Songklanarin Journal of Science and Technology 17(2):115-20.


Article Metrics

Abstract view : 26 times
PDF - 23 times


  • There are currently no refbacks.

Copyright (c) 2016 PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science)


Alamat Redaksi
Redaksi Planta Tropika: Jurnal Agrosains (Journal of Agro Science)
Universitas Muhammadiyah Yogyakarta
Jl. Ring Road Selatan, Tamantirto, Kasihan, Bantul
Telp (0274) 387646 psw 224.
E-ISSN: 2528-7079
p-ISSN: 0216-499X


Creative Commons License
Planta Tropika: Jurnal Agrosains (Journal of Agro Science) is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.