Effects of Arbuscular Mycorrhiza-Enriched Bio-compost and Organic Fertilizer on Reducing Heavy Metal Absorption in Shallots
DOI:
https://doi.org/10.18196/pt.v13i1.21684Keywords:
Colonization, Endomycorrhizae, Organic fertilizer, Spice plantAbstract
Shallots have physiological and pharmacological effects on the human body and have been cultivated intensively using bio-compost, which might be contaminated with heavy metals. The main objective of this study is to enrich arbuscular mycorrhizal fungi (AMF) in bio-compost and add biological organic fertilizer (BOF) to reduce the absorption of heavy metals in shallot bulbs. The study was conducted in Enrekang District, South Sulawesi, where the components of heavy metal content in plant organs and the level of infection of AMF in plant roots were observed. The study was a filed research arranged in a Randomized Block Design with five treatments, including shallots cultivation method by indigenous farmers/control(B0) and using various doses of bio-compost enriched with AM fungi of 100kg plot-1(B1); 200kg plot-1(B2); 100kg plot-1+BOF(B3); and 200kg plot-1+BOF). The results showed that bio-compost enriched with AMF at a dose of 200 kg plot-1 and 100kg plot-1+BOF could increase Cr, Cu, and Pb accumulation in roots and leaf, as well as reduce metal accumulation in shallot bulbs, with the level of AMF infection classified as very high. The novelty of this study is that organic fertilizer enriched with AMF can reduce the absorption of heavy metals accumulated in shallot bulbs.
References
Ali, A. S. M., Ahmed, H. A. M., Emara, H. A. E. A., Janjua, M. N., & Alhafez, N. (2019). Estimation and bio-availability of toxic metals between soils and plants. Polish Journal of Environmental Studies, 28(1). https://doi.org/10.15244/pjoes/81690
Alloway, B. J. (2013). Heavy Metals in Soil, Trace Metals and Metalloids in Soils and their Bioavailability (I. Colbeck, R. L. Crawford, & W. Salomons (eds.); Third Edit). Springer Dordrecht Heidelberg. https://doi.org/10.1007/978-94-007-4470-7
Amare, G. (2020). Review on Mineral Nutrition of Onion (Allium cepa L ). The Open Biotechnology Journal, 14(December 2020), 134–144. https://doi.org/10.2174/1874070702014010134
Angulo-Bejarano, P. I., Puente-Rivera, J., & Cruz-Ortega, R. (2021). Metal and metalloid toxicity in plants: An overview on molecular aspects. Plants, 10(4), 1–28. https://doi.org/10.3390/plants10040635
Aryanta, I. W. R. (2019). Bawang merah dan manfaatnya bagi kesehatan. Jurnal Widya Kesehatan, 1(1), 1–7. https://ejournal.unhi.ac.id
Balali-mood, M., Naseri, K., Tahergorabi, Z., Khazdair, M. R., & Sadeghi, M. (2021). Toxic Mechanisms of Five Heavy Metals : Mercury , Lead , Chromium , Cadmium , and Arsenic. Frontiers in Pharmacology, 12(April), 1–19. https://doi.org/10.3389/fphar.2021.643972
Bamba, B. S. B., Komenan, A. C. A., Kouassi, K. K. P., & Soro, D. (2020). Effects of onion bulb processing conditions on drying characteristics, physicochemical and functional properties profile of onion (Allium cepa L.) powder. Journal of Food Science, 85(10), 3345–3354. https://doi.org/10.1111/1750-3841.15415
Begum, N., Qin, C., Ahanger, M. A., Raza, S., Khan, M. I., Ashraf, M., Ahmed, N., & Zhang, L. (2019). Role of Arbuscular Mycorrhizal Fungi in Plant Growth Regulation: Implications in Abiotic Stress Tolerance. Frontiers in Plant Science, 10(1068), 1–15. https://doi.org/10.3389/fpls.2019.01068
Bhat, B. A., Islam, S. T., Ali, A., Sheikh, B. A., Tariq, L., Islam, S. U., & Dar, T. U. H. (2020). Plant Micronutrients: Role of Micronutrients in Secondary Metabolism of Plants (Eds. T. Aftab & K. R. Hakeem). Springer Natur Switzerland. https://doi.org/10.1007/978-3-030-49856-6
Boorboori, M. R., & Zhang, H. (2022). Arbuscular Mycorrhizal Fungi Are an Influential Factor in Improving the Phytoremediation of Arsenic , Cadmium , Lead , and Chromium. Journal of Fungi, 8, 176. https://doi.org/https://doi.org/10.3390/jof8020176
Briffa, J., Sinagra, E., & Blundell, R. (2020a). Heavy metal pollution in the environment and their toxicological effects on humans. Heliyon, 6(9), e04691. https://doi.org/10.1016/j.heliyon.2020.e04691
Briffa, J., Sinagra, E., & Blundell, R. (2020b). Heliyon Heavy metal pollution in the environment and their toxicological effects on humans. Heliyon, 6(August), e04691. https://doi.org/10.1016/j.heliyon.2020.e04691
Brundrett, M., Bougher, N., Dell, B., Grove, T., & Malajczuk, N. (1996). Working with Mycorrhizas in Forestry and Agriculture Mycorrhizas of Australian Plants View project Banksia Woodland Restoration Project View project (P. Lynch (ed.); Issue June 1982). The Australian Centre for International Agricultural Research (AClAR). https://www.researchgate.net/publication/227365112
Chot, E., & Reddy, M. S. (2022). Role of Ectomycorrhizal Symbiosis Behind the Host Plants Ameliorated Tolerance Against Heavy Metal Stress. Frontiers in Microbiology, 13(March). https://doi.org/10.3389/fmicb.2022.855473
Chulikavit, N., Huynh, T., Khatibi, A., Das, R., & Kandare, E. (2023). Thermal degradation and flame spread characteristics of epoxy polymer composites incorporating mycelium. Scientific Reports, 13(1), 1–19. https://doi.org/10.1038/s41598-023-45097-0
Dewi, T., Martono, E., Hanudin, E., & Harini, R. (2021). Heavy metals contamination assessment in agricultural soil for shallot in Wanasari, Brebes Regency, Central Java Province. IOP Conference Series: Earth and Environmental Science, 752(1), 1–8. https://doi.org/10.1088/1755-1315/752/1/012056
Dhalaria, R., Kumar, D., Kumar, H., Nepovimova, E., Kuca, K., Islam, M. T., & Verma, R. (2020). Arbuscular mycorrhizal fungi as potential agents in ameliorating heavy metal stress in plants. Agronomy, 10(6), 1–22. https://doi.org/10.3390/agronomy10060815
Doyama, K., Yamaji, K., Haruma, T., & Id, A. I. (2021). Zn tolerance in the evergreen shrub , Aucuba japonica , naturally growing at a mine site : Cell wall immobilization , aucubin production , and Zn adsorption on fungal mycelia. PLOS ONE, 16(9), e0257690. https://doi.org/10.1371/journal.pone.0257690
Ejaz, U., Khan, S. M., Khalid, N., Ahmad, Z., Jehangir, S., Fatima Rizvi, Z., Lho, L. H., Han, H., & Raposo, A. (2023). Detoxifying the heavy metals: a multipronged study of tolerance strategies against heavy metals toxicity in plants. Frontiers in Plant Science, 14(May), 1–17. https://doi.org/10.3389/fpls.2023.1154571
El- Shabasy, sara, elbasuny, A., & talaha, N. (2023). Effect of Different Phosphate Fertilizers on the Availability of some Heavy Metals in El-Gabal El Asfer Soil under Maize and Wheat Cropping System. Egyptian Journal of Soil Science, 63(3), 267–275. https://doi.org/10.21608/ejss.2023.196968.1574
Febrita, E. (2020). Analysis of Concentration of Heavy Metal (Pb) and Cadmium (Cd) In Waterfish (Eichhornia crassipes) in Indragiri River, Riau. International Journal of Ecophysiology, 2(1), 24–33. https://doi.org/10.32734/ijoep.v2i1.3985
Giannakoula, A., Therios, I., & Chatzissavvidis, C. (2021). Effect of Lead and Copper on Photosynthetic Apparatus in Citrus (Citrus aurantium L.) Plants. The Role of Antioxidants in Oxidative Damage as a Response to Heavy Metal Stress. Plants, 10(1), 155. https://doi.org/10.3390/plants10010155
Gong, X., & Tian, D. Q. (2019). Study on the effect mechanism of Arbuscular Mycorrhiza on the absorption of heavy metal elements in soil by plants. IOP Conference Series: Earth and Environmental Science, 267(5), 1–7. https://doi.org/10.1088/1755-1315/267/5/052064
Haider, F. U., Liqun, C., Coulter, J. A., Cheema, S. A., Wu, J., Zhang, R., Wenjun, M., & Farooq, M. (2021). Ecotoxicology and Environmental Safety Cadmium toxicity in plants: Impacts and remediation strategies. Ecotoxicology and Environmental Safety, 211, 111887. https://doi.org/10.1016/j.ecoenv.2020.111887
Hamidah, Putri, D., & Purnobasuki, H. (2020). Correlation of lead ( Pb ) content towards the number of stomata on the plant Dracaena marginata tricolor in some places of Surabaya City. International Conference on Sustainability Science and Management: Advanced Technology in Environmental Research (CORECT-IJJSS 2019), 153(2020), 1–7. https://doi.org/10.1051/e3sconf/202015302002
Hardiyanti, Y., Fahruddin, F., & Paulina, T. (2020). Accumulation Of Heavy Metal Lead (Pb) And Effect Of Stomates Number On Green Champa Leaves (Polyalthia longifolia) In Industrial Area Of Makassar City. International Journal of Applied Biology, 4(2), 1–8.
Hawayanti, E., Aminah, I. S., Syahputra, N., Moulyohadi, Y., & Astuti, D. T. (2021). Community Empowerment Through Red Onion Cultivation with Polybags on 1 Ilir Urban Village, Palembang City. International Journal of Community Engagement, 2(1), 62–67. https://doi.org/10.32502/altifani.v2i1.3844
Huey, C. J., Gopinath, S. C. B., Uda, M. N. A., Zulhaimi, H. I., Jaafar, M. N., Kasim, F. H., & Yaakub, A. R. W. (2020). Mycorrhiza: a natural resource assists plant growth under varied soil conditions. 3 Biotech, 10(204), 1–9. https://doi.org/10.1007/s13205-020-02188-3
Husna, Tuheteru, F. D., & Arif, A. (2021). Arbuscular mycorrhizal fungi to enhance the growth of tropical endangered species pterocarpus indicus and pericopsis mooniana in post gold mine field in southeast sulawesi, Indonesia. Biodiversitas, 22(9), 3844–3853. https://doi.org/10.13057/biodiv/d220930
Kabata-Pendias, A., & Pendias, H. (2011). Trace Elements in Soils and Plants (4th Editio). CRC Press, Boca Raton.
Li, W., Chen, K., Li, Q., Tang, Y., Jiang, Y., & Su, Y. (2023). Effects of Arbuscular Mycorrhizal Fungi on Alleviating Cadmium Stress in Medicago truncatula Gaertn. Plants, 12(3), 1–15. https://doi.org/10.3390/plants12030547
Lindawati, E., Khairuddin, Nurhaeni, Ruslan, Ridhay, A., Sosidi, H., Rahim, E. A., & Syamsuddin. (2023). Analysis of Cadmium (Cd) in Palu local shallots (Allium cepa L. var. aggregatum) at various Llocations and planting ages. Jurnal Riset Kimia, 9(1), 70–76. https://doi.org/10.22487/kovalen.2023.v9.i1.16287
Marefati, N., Ghorani, V., Shakeri, F., Boskabady, M., Kianian, F., Rezaee, R., & Boskabady, M. H. (2021). A review of anti-inflammatory, antioxidant, and immunomodulatory effects of Allium cepa and its main constituents. Pharmaceutical Biology, 59(1), 287–302. https://doi.org/10.1080/13880209.2021.1874028
Ministry Of State for Population and Environment republic of Indonesia and Dalhousie University Canada. (1992). Environmental Management in Indonesia. Report on Soil Quality Standards for Indonesia. In (interim report).
Muchoka, J. P., Mugendi, D. N., Njiruh, P. N., Onyari, C., Mbugua, P. K., & Njeru, E. M. (2020). Arbuscular Mycorrhizal Fungi Colonization in the Rhizosphere of Aspilia pluriseta Schweif . ext Schweif in the Semiarid Eastern Kenya. Air, Soil and Water Research, 13, 1–10. https://doi.org/10.1177/1178622120969190
Mustofa, F. I., Rahmawati, N., & Aminullah. (2020). Medicinal plants and practices of Rongkong Traditional Healers in South Sulawesi, Indonesia. Biodiversitas, 21(2), 642–651. https://doi.org/10.13057/biodiv/d210229
Nur, S., Suwarto, S., Saparso, S., & Djatmiko, H. A. (2020). Morfo-Physiological Response of Three Shallot Varieties on Water Surface Variation Level. International Journal of Applied Science, 3(2), p1. https://doi.org/10.30560/ijas.v3n2p1
Nurlaili, R. A., Rahayu, Y. S., & Dewi, S. K. (2021). Pengaruh Mikoriza Vesikular Arbuskular (MVA) dan Silika (Si) terhadap Pertumbuhan Tanaman Brassica juncea pada Tanah Tercemar Kadmium (Cd). LenteraBio : Berkala Ilmiah Biologi, 9(3), 185–193. https://doi.org/10.26740/lenterabio.v9n3.p185-193
Pinto, K. R., Meza-Contreras, V., Alegrre-Orihuela, J. C., & Reategui-Romero, W. (2020). Bioavailability and Solubility of Heavy Metals and Trace Elements during Composting of Cow Manure and Tree Litter. Applied and Environmental Soil Science, 2020, 1–20. https://doi.org/https://doi.org/10.1155/2020/5680169 Research
Priyadi, S., Harieni, S., Soemarah, T., Utami, D. S., & Haryuni. (2021). Good Agricultural Practices Shallot (Allium ascalonicum) with Cow Manure, Food Safety Review From Heavy Metal Content Aspects. JURNAL ILMIAH AGRINECA, 21(1), 20-24. https://doi.org/10.36728/afp.v21i1.1318
Prosanti, D. A., Prasetya, B., & Soemarno, S. (2023). Aplikasi Lubang Resapan Biopori Berkompos Di Kebun Kopi Meningkatkan Jumlah Spora Mikoriza Arbuskula Dan Koloni Akar. Jurnal Tanah Dan Sumberdaya Lahan, 10(2), 341–351. https://doi.org/10.21776/ub.jtsl.2023.010.2.18
Putra, B., Warly, L., Evitayani, & Utama, B. P. (2022). The role of arbuscular mycorrhizal fungi in phytoremediation of heavy metals and their effect on the growth of Pennisetum purpureum cv. Mott on gold mine tailings in Muara Bungo, Jambi, Indonesia. Biodiversitas, 23(1), 478–485. https://doi.org/10.13057/biodiv/d230151
Rajapakse, S., & Miller, J. C. (1992). Methods for Studying Vesicular-arbuscular Mycorrhizal Root Colonization and Related Root Physical Properties. Methods in Microbiology, 24, 301–316. https://doi.org/10.1016/S0580-9517(08)70098-9
Ramísio, P. J., Bento, F., Geraldo, D., Andrade, O., & Bettencourt, A. P. (2023). Evaluation of Municipal Waste Compost in Relation to the Environmental Retention of Heavy Metals. Sustainability, 15(23), 16395. https://doi.org/10.3390/su152316395
Rasman, & Hasmayani. (2018). Factors That Influence Ethical Content (Pb) In Red On (Allium Cepa) In Pekalobean Village, Enrekang District. Jurnal Sulolipu, 18(I), 47–52.
Riaz, M., Kamran, M., Fang, Y., Wang, Q., Cao, H., Yang, G., Deng, L., Wang, Y., Zhou, Y., Anastopoulos, I., & Wang, X. (2021). Arbuscular mycorrhizal fungi-induced mitigation of heavy metal phytotoxicity in metal contaminated soils : A critical review. Journal of Hazardous Materials, 402, 123919. https://doi.org/10.1016/j.jhazmat.2020.123919
Robifahmi, N., Anas, I., Setiadi, Y., Ishak, & Citraresmini, A. (2020). Uji Kemampuan Mikoriza dalam Meningkatkan Serapan P, Efisiensi Pupuk dan Hasil Tanaman Sorgum pada Tanah Latosol Menggunakan Teknik Isotop 32P. Jurnal Ilmiah Aplikasi Isotop Dan Radiasi, 16(1), 50.
Ruhban, A., & Kurniati. (2017). Kandungan Logam Berat Timbal (Pb) Dalam Residu Pestisida Pada Tanah, Air Dan Bawang Merah Di Desa Salu Dewata Kecamatan Anggeraja Kabupaten Enrekang. Jurnal Sulolipu, 17(2), 19–24.
Saparso, Kartini, & Apriliyanto, I. D. (2021). Growth and yield of shallots (Allium ascalonicum L.) Lokananta in various doses of nitrogen fertilizers and number of plant per hole in coastal sandy land. IOP Conference Series: Earth and Environmental Science, 653(1). https://doi.org/10.1088/1755-1315/653/1/012145
Setiawandari, Istiqomah, & Ximenes, M. D. S. (2021). Innovation of Shallot (Allium ascalonicum L) Essential Oil for Fever Reduction in Infants Post-DTaP Immunization. Journal of Health Sci Ence, 14(3), 187–195.
Srivastava, D., Tiwari, M., Dutta, P., Singh, P., & Chawda, K. (2021). Chromium Stress in Plants : Toxicity , Tolerance and Phytoremediation. MDPI, 13(9), 1–20.
Subiksa, I. G. M., Purnomo, J., & Suastika, I. W. (2020). Effects of “ Peat Fertilizer ” on Heavy Metal Leaching and Uptake by Maize on Peat Soil. Jurnal Tanah Dan Iklim, 44(1), 19–29. http://dx.doi.org/10.21082/jti.v44n1.2020.19-29
Zafran, Mahardika, K., Mastuti, I., & Nadyasari, K. R. (2021). The Efectiviness of Shallot (Allium cepa) , Garlic (Allium sativum) , And Leek (Allium cepa) Extract Against Marine Leech Hirudinea (Zeylanicobdella arugamensis) In Vitro. Fishering Journal, 3(1), 44–55. https://fisheries.hangtuah.ac.id/
Zulfiqar, U., Farooq, M., Hussain, S., Maqsood, M., Hussain, M., Ishfaq, M., Ahmad, M., & Anjum, M. Z. (2019). Lead toxicity in plants : Impacts and remediation. Journal of Environmental Management, 250, 109557. https://doi.org/https://doi.org/10.1016/j.jenvman.2019.109557
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