Callus Induction and Differentiation on Melon From In Vitro Culture with The Addition of Indole Acetic Acid and Benzyl Amino Purine Growth Regulator

Wiwit Probowati, Budi Setiadi Daryono


Melon plants (Cucumis melo L.) are susceptible to disease attacks primarily caused by viruses. One of the viruses often attacking melon plants is Cucumber mosaic virus (CMV). Previous studies have shown that melon callus produced from in vitro culture is detected to have CMV resistance gene (Creb-2). However, the trait of resistance to CMV in that melon (F2) is still segregated. To know the stability of the Creb-2 gene, further research is needed to detect the gene in the differentiated callus. This study aims to  callus induce and callus differentiate melon. Firstly, melon seeds were grown on ¼ MS medium with addition of 2 mg / L 2.4-Dichlorophenoxyacetate and 1 mg / L BAP (Benzyl Amino Purine) to grow callus. Secondly, the callus was differentiated in MS medium with the addition of 2,4-D , BAP and IAA in different doses. 0 mg / L; 0.01 mg / L 2.4-D and 0.1 mg / L BAP, and MS medium with 0.01 mg / L; 0.1 mg / L; 1 mg / L IAA (Iodole Acetic Acid) and 0.1 mg / L BAP. The results showed that callus was successfully differentiated into root and it grew optimally in MS medium with 0.01 mg / L 2.4-D: 0.1 mg / L BAP and 1 mg / L IAA: 0.1 mg / L BAP . Therefore the roots can be used as isolation samples and DNA amplification to detect stability of the Creb-2 gene.


Cucumis melo L.; Callus; CMV; in vitro

Full Text:



Daryono, B.S., S. Somowiyarjo, and K.T. Natsuaki, 2003. New source of resistance to Cucumber mosaic virus in melon. SABRAO Journal of Breeding and Genetics. 35: 19-26.

Daryono, B.S., K. Wakui, and K.T. Natsuaki.2005. Isolation and characterization of molecular markers linked to CMV-B2 resistance gene in melon.J.Phytopathol. 71: 278.

Daryono, B. S. 2009. Development and Application of Melon Resistance Gene Analog (MRGA) Markers for Detection of Virus Resistance Gene in Melon (Unpublished). Faculty of Biology Gadjah Mada University. Yogyakarta.

Daryono, B.S. dan S.D. Maryanto. 2017. Keanekaragaman dan potensi Sumber Daya Genetik Melon. UGM Press. Yogyakarta.

George, E. F., and P.D. Sherrington. 2004. Plant Propagation by Tissue Culture. Exegetics Ltd. England.

Hirai, S., and Y. Amemiya.1989. Studies on the resistance of melon cultivars to cucumber mosaic virus (I) virus multiplication in leaves or mesophyll protoplasts from a susceptible and resistant cultivars. Ann. Phytopath. Soc. Japan 55: 458-465.

Hirai, S., and Y. Amemiya. 1990. Studies on the resistance of melon cultivars to cucumber mosaic virus (II) RNA profiles of virus multiplied in resistant and susceptible cultivars. Ann. Phytopath. Soc. Japan 56: 39-46.

Larkin, S. and W. Snowcroft. 1981. Somaclonal variation-a novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet 60:197–214.

Listiawan, D. A., 2009. Deteksi Gen Pengkode Sifat Ketahanan Terhadap Powdery Mildew (Podosphaera xanthii (Castagne) U. Braun & N. Shishkoff) pada Melon (Cucumis melo L.) dengan Penanda Molekuler Sequence Characterized Amplified Region (SCARs). Skripsi. Fakultas Biologi. Universitas Gadjah Mada. Yogyakarta.

Machado, S. R., M. E.M. Estelita, and E. A. Gregorio. 2000. Ultrastructure of the intercellular protuberances in leaves of Paepalanthus Superbus. Ruhl (Eriocaulaceae). Revista Brasileira de Botânica. 23 (4): 100-111.

Moreno,V., M. Garcia-Sogo, I. Granell, B. Garcia-Sogo, and L. A. Roig. 1985. Plant regeneration from calli of melon (Cucumis melo L.,cv. 'Amarillo Oro'). Plant Cell Tissue Organ Culture. 5:139-146. 139.

Neumann, K.H., A. Kumar, and J. Imani. 2009. Plant Cell and Tissue Culture-A Tool in Biotechnology. Springer-Verlag Berlin Heidelberg. Jerman. pp.75-83. technology-Basic-and-Aplication-274066.html

Rahmat. 2009. Isolasi dan Amplifikasi Gen Ketahanan terhadap Cucumber Mosaic Virus (Creb-2) pada Sel-Sel Kalus Melon (Cucumis meloL.) Hasil Kultur In Vitro. Skripsi. Fakultas Biologi. Universitas Gadjah Mada. Yogyakarta.

Selvaraj, N., A. Vasudevan, M. Manickavasagam, and A. Ganapathi. 2006. In vitro organogenesis and plant formation in cucumber. Biologia Plantarum 50:123-126.

Somowiyarjo, S., N. Sako, and K. Tomaru. 1993. The use of dot immunobinding assay for detecting cucurbit viruses in Yogyakarta. In: Production of virus-free tropical crops. Editor : Tomaru, K. and Natsuaki, K.T. NODAI Center for International Programs. Tokyo University of Agriculture. Tokyo. pp. 3-11.

Tabei, Y., T. Kanno, and T. Nihio. 1991. Regulation of organogenesis and somatic embryogenesis by auxyn in melon, Cucumis melo L. Plant cell report. Springer-Verlag. 10:225-229.

Wetter, L. R., and F. Constabel. 2011. Metode Kultur Jaringan Tumbuhan. Edisi ke-4. Penerbit ITB. Bandung. pp. 1-3, 14,18-20.


Article Metrics

Abstract view : 68 times
PDF - 67 times


  • There are currently no refbacks.

Copyright (c) 2018 Universitas Muhammadiyah Yogyakarta

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.


Alamat Redaksi
Redaksi Planta Tropika: Jurnal Agrosains (Journal of Agro Science)
Universitas Muhammadiyah Yogyakarta
Jl. Ring Road Selatan, Tamantirto, Kasihan, Bantul
Telp (0274) 387646 psw 224.
E-ISSN: 2528-7079
p-ISSN: 0216-499X


Creative Commons License
Planta Tropika: Jurnal Agrosains (Journal of Agro Science) is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.