Exploration of Arbuscular Mycorrhizal Fungi from Sugarcane Rhizosphere in Marginal Land

Authors

  • Zahraeni Kumalawati Pangkep State Polytechnic of Agriculture, Pangkep, 90655,
  • Sri Muliani Pangkep State Polytechnic of Agriculture, Pangkep, 90655,
  • Asmawati Asmawati Pangkep State Polytechnic of Agriculture, Pangkep, 90655,
  • Kafrawi Kafrawi Pangkep State Polytechnic of Agriculture, Pangkep, 90655,
  • Yunus Musa Hasanuddin University, Makassar, 90245

DOI:

https://doi.org/10.18196/pt.v9i2.4026

Keywords:

Arbuscular Mycorrhizal Fungi, rhizosphere, Sugarcane

Abstract

The exploration of arbuscular mycorrhiza fungi from sugarcane plantation in marginal land in South Sulawesi was carried out to find the source of inoculums showing effective infection. Soil samples were taken from four area with different characteristic of marginal land, namely land with low organic matter content, clay texture, limited irrigation, and undulating land. Mycorrhizae contained in the soil samples were then observed, and the spores obtained were used as the source of isolation by a single spore culture. The mycorrhizal spores were isolated by wet sieving and centrifugation method with 48% sucrose, which were observed under a compound microscope for spore details (100-1000x). Sugarcane root samples were taken to observe mycorrhizal infection in sugarcane root tissue by root staining method. The results of the study showed that the greatest diversity of mycorrhizal genera was found in soil samples of Jambua Block (Glomus, Gigaspora, and Sclerocistis) and AJ-5 Block area (Glomus, Acaulospora, and Sclerocistis). Single-spore isolates obtained were Glomus sp. and Acaulospora sp. Infection test result on four sugarcane varieties commonly grown in Takalar Sugar Factory showed that infectivity of mycorrhizal isolate of Acaulospora sp. was the highest (75%) and significantly different (LSD’test, p <0,05) compared to that of Glomus sp. (66%).

References

Álvarez-Sánchez, J., Johnson N.C., Antoninka A., Chaudhary V.B., Lau M.K., Owen S.M., Sánchez-Gallen I., Guadarrama P. & Castillo S., 2012. Large-scale Diversity Patterns in Spore Communities of Arbuscular Mycorrhizal Fungi (30-47) in Mycorrhiza: Occurrence in Natural and Restored, Eds : Pagano M., 2012. Nova Science Publishers, Inc.

Arifin, B., 2008. Ekonomi Swasembada Gula Indonesia. Economic Review. No. 21:1 – 12.

Begum N., Qin C., Ahanger M.A., Raza S., Khan M.I., Ashraf M, Ahmed N. & Zhang L., 2019. Role of Arbuscular Mycorrhizal Fungi in Plant Growth Regulation : Implications in Abiotic Stress. Tolerance. Front. Plant Sci. 10:1068.

Bouyoucos, C.J. 1962. Hydrometer method improved for making particle size analysis of soils. Agronomy Journal 54:464-465.

Brundrett M., Bougher N., Dell B., Grove T., & Malajczuk N., 1996. Working with Mycorrhizas in Forestry and Agriculture. ACIAR Monograph 32, Canberra, Australia.

Castillo, C.G., Borie F., Oehl F., & Sieverding E., 2016. Arbuscular Mycorrhizal Fungi Biodiversity: Prospecting in Southern-Central zone of Chile, A review. Journal of Soil Science and Plant Nutrition. 16(2):400-422.

Chen, M., Arato M., Borghi L., Nouri E. & Reinhardt D., 2018. Beneficial Services of Arbuscular Mycorrhizal Fungi – From Ecology to Application. Front. Plant Sci. 9:1270.

Datta, P & Kulkarni M., 2012. Arbuscular Mycorrhizal Fungal Diversity in Sugarcane Rhizosphere in Relation with Soil Properties. Not Sci Biol, 4(1): 66-74.

Delvian, 2006. Koleksi Isolat Cendawan Mikoriza Arbuskula asal Pantai (Karya Tulis). Jurusan Kehutanan Fakultas Pertanian Universitas Sumatera Utara, Medan.

Direktorat Jenderal Perkebunan, 2012. Percepatan Pelaksanaan Kegiatan 2012 untuk Suksesnya Swasembada Gula 2014. Kementrian Pertanian, Jakarta.

Eviati & Sulaeman, 2009. Petunjuk Teknis Analisis Kimia Tanah, Tanaman, Air, dan Pupuk Balai Penelitian Tanah, Bogor.pp234.

Fahad S., Bajwa A.A., Nazir U., Anjum S.A., Farooq A., Zohaib A., Sadia S., Nasim W., Adkins, Saud S, Ihsan M.Z, Alharby H., Wu C., Wang D., & Huang J., 2017. Crop production under drought and heat stress: plant responses and management options. Front. Plant. Sci. 8: 1147.

Giovanetti M. & Mosse B., 1980. An Evaluation of Techniques to measure Vesicular-arbuscular Mycorrhiza Infection in Roots. New Phytol. 84:489-500.

Hajek, B.F., Adams F, & Cope J.T.. 1972. Rapid determination of exchangeable bases, acidity and cation exchange capacity. Soil Sci. Soc. Am. Proc. 36: 436 - 438.Harrison M.J., 1997. The arbuscular Mycorrhizal Symbiosis: an Underground Association. Trends Plant Sci 2:54–60.

Ismayanti, W., Toekidjo & Hadisutrisno B., 2013. Pertumbuhan dan Tanggapan terhadap Penyakit Karat (Puccinia kuehnii) Sembilan Klon Tebu (Saccharum officinarum L.) yang Diinfeksi Jamur Mikoriza Arbuscular. Vegetalika 2(4): 75-87.

Jakhar, S.R., Kumar S., Jangir C.K. & Meena R.S., 2017. The Role of Mycorrhizal Relationship in Sustainable Manner Towards Plant Growth and Soil Fertility. Indian Journal of Agriculture and Allied Sciences 3(4):19-24.

Jamal, S.F., Cadet P., Rutherford R.S., & Straker C.J., 2004. Effect of Mycorrhiza on the Nutrient Uptake of Sugarcane. Proc. South African Sugar Association , South Africa. pp 78.

Jones M.D. & Smith S.E., 2004. Exploring functional definitions of mycorrhizas : Are mycorrhizas always mutualisms? Can. J. Bot. 82: 1089–1109.

Kelly, R.M., Edwards D.G, Thompson J.P., and Magarey R.C., 2005. Growth Responses of Sugarcane to Mycorrhizal spore Density and Phosporus Rate. Australian J. Agri Res 56 :1405-1413.

Kim, S.J., Eo J.K, Lee E.H., Park H. & Eom A.H., 2017. Effects of Arbuscular Mycorrhizal Fungi and Soil Conditions on Crop Plant Growth. Mycobiology 45(1): 20-24.

Kormanik, P. P. & McGraw A. C.. 1982. Quantification of Vesicular-arbuscular Mycorrhizae in Plant Roots. In Methods and Principles of Mycorrhizal Research. Ed. N.C.

Kramadibrata, K., Riyanti E.I., & Simanungkalit R.D.M., 1995. Arbuscular Mycorrhizal Fungi From the Rhizosphere of Soybean Crops in Lampung and West Java. Biotropia, 8: 30-38.

Kumalawati, Z., Musa Y., Amin N., Asrul L., & Ridwan I., 2014. Exploration of Arbuscular Mycorrhizal Fungi from Sugarcane Rhizosphere in South Sulawesi. International Journal of Scientific & Technology Research 3(1):201:203.

Latef, A.A.H.A., Hashem A., Alqarawi A.A, Rasool S., Allah E.F.A, Egamberdieva D., Jan S., Anjum N.A. & Ahmad P., 2016. Arbuscular Mycorrhizal Symbiosis and Abiotic Stress in Plants: A Review. J. Plant Biol. 59:407-426.

Lee, E.H., Eo J.K., Ka K.H., & Eom A.H., 2013. Diversity of Arbuscular Mycorrhizal Fungi and Their Roles in Ecosystems. Mycobiology 41(3): 121–125.

Leovini H., Kastono D. & Widada J., 2014. Pengaruh Pemberian Jamur Mikoriza Arbuskular, Jenis Pupuk Fosfat dan Takaran Kompos terhadap Pertumbuhan Bibit Tebu (Saccharum officinarum l.) pada Media Pasir Pantai. Vegetalika 3(1):102-115.

Linderman, R.G. 2000. Effects of mycorrhizas on plant tolerance to diseases. Pages 345–365 in Y. Kapulnik and D. D. Douds, Jr., eds. Arbuscular mycorrhizas: Physiology and function. Kluwer Academic Press, Dordrecht, The Netherlands.

Lone, R., Shuab R., Khan S., Ahmad J. , & Koul K.K., 2017. Arbuscular Mycorrhizal Fungi for Sustainable Agriculture. In Probiotics and Plant Health. Springer, Singapore. pp 553-577.

Mirshad, P. P., & Puthur, J. T., 2016. Arbuscular Mycorrhizal Association Enhances Drought Tolerance Potential of Promising Bioenergy Grass Saccharum arundinaceum, Retz. Environ. Monit. Assess. 188, 425.

Mutert, E., Dierolf T., & Fairhurst. 2000. Soil fertility kit: a toolkit for acid upland soil fertility management in Southeast Asia. PPI: Singapore.

Neumann, E. & George E., 2010. Nutrient Uptake: The Arbuscular Mycorriza Fungal Symbiosis as a Plant Nutrient Acquisition Strategy. Pages 137-168 in Y. Kapulnik and D. D. Douds, Jr., eds. Arbuscular mycorrhizas: Physiology and function. Kluwer Academic Press, Dordrecht, The Netherlands.

Nurhalimah, S., Nurhatika S., & Muhibuddin A., 2014. Eksplorasi Mikoriza Vesikular Arbuskular (MVA) Indigenous pada Tanah Regosol di Pamekasan, Madura Jurnal Sains dan Seni Pomits 3(1):30-34.

Nurhalisyah & Rahmad D., 2012. Identifikasi fungi mikoriza arbuskular di lahan tebu PTPN XIV serta efektifitasnya untuk meningkatkan serapan fosfat dalam menunjang produksi tebu. Jurnal Agrisistem Seri Hayati 8 (2): 62-69.

Olsen, S.R., Cole C.V., Watanabe F.S., & Dean L.A., 1954. Estimation of available P in soils by extraction with sodium bicarbonate. USDA cir.No 939.Pavithra, D., and Yapa, N., 2018. Arbuscular Mycorrhizal Fungi Inoculation Enhances Drought Stress Tolerance of Plants. Ground Water Sust. Dev. 7:490–494.

Pusat Informasi BUMN Perkebunan, 2012. ProfilePTPNXIVMakassar (http://www.lpp.ac.id/ptpn) Access date: September 2016.

Rayment, G.E. & Higginson F.R., 1992. Australian laboratory handbook of soil and water chemicals methods. Australian soil and land survey handbook. Inkata Press, Melbourne, Sydney.

Rismaneswati, 2005. Analisis Kesesuaian Lahan Sebagai Dasar Optimalisasi Penggunaan Sumberdaya Lahan Perkebunan Tebu (Studi Kasus Takalar) (Thesis). Program Pascasarjana, Unhas, Makassar.p83.

Rokni, N. & Goltapeth E.M., 2011. Diversity of Arbuscular Mycorrhizal Fungi associated with common sugarcane varieties in Iran. J. of Agri Tech. 7(4): 1017-1022.

Rossetto R., Dias F.L.F., Vitti A.C. & Cantarella H, 2014. Fertility maintenance and soil recovery in sugarcane crops, p.381-404. In Cortez L.A.B (Coord.). Sugarcane bioethanol —R&D for Productivity and Sustainability, São Paulo: Blücher E.(eds). Sugarcanebioethanol_38.

Sabatier D., Martin J.F., Chiroleu F., Roussel C., Letourmy P, Antwerpen R.V., Gabrielle B. & Ney B., 2015. Optimization of sugarcane farming as a multipurpose crop for energy and food production. GCB Bioenergy 7: 40–56.

Setiadi, Y. & Setiawan A., 2011. Studi Status Fungi Mikoriza Arbuskula di Areal Rehabilitasi Pasca Penambangan Nikel (Studi Kasus PT INCO Tbk. Sorowako, Sulawesi Selatan). J. Silvikultur Tropika 3(1):88-95.

Sjoberg, J. (2005). Arbuscular Mycorrhiza Fungi. Occurence in Sweden and Interaction with a Plant Pathogenic Fungus in Barley. Acta Universitais Agriculturae Sueciae. Uppsala.

Sieverding, E., (1991). Vesicular Arbuskular Mycorrhiza: Managemenent in Tropical Agrosystem. Germany, GTZ GmbH.

Silva, G.A.E., Siqueira J.O., Stürmer S.L., & Moreira F.M.S., 2018. Effectiveness of Arbuscular Mycorrhizal Fungal Isolates from the Land Uses of Amazon Region in Symbiosis with Cowpea. An. Acad. Bras. Ciênc.90 (1):357-371.

Soenartiningsih, 2007. Peranan Jamur Mikoriza Arbuskular dalam Mengendalikan Penyakit Busuk Pelepah (Rhizoctonia solani) pada Jagung. Disertasi Univ. Gadjah Mada, Yogyakarta.

Sugito, Y., Nuraini Y., & Nihayati E.. 1995. Sistem Pertanian Organik. Fakultas Pertanian Universitas Brawijaya. pp84.

Sulaiman A.A., Sulaeman Y, Mustikasari N., Nursyamsi D. & Syakir A.M. Increasing sugar production in indonesia through land suitability analysis and sugar mill restructuring. Land 8 (61):1-17.

Sulistiono W., Taryono, Yudono P., & Irham, 2017. Early-Arbuscular Mycorrhizal Fungi-Application Improved Physiological Performances of Sugarcane Seedling and Further Growth in the Dry Land . Journal of Agricultural Science 9(4):95-108.

Surendran, U. & Vani D., 2013. Influence of Arbuscular Mycorrhizal Fungi in Sugarcane Productivity Under Semiarid Tropical Agro ecosystem in India. International Journal of Plant Production 7(2):269-277.

Suresh N. & Nelson R., 2015. Diversity of Arbuscular Mycorrhizal fungi (AMF) in the rhizosphere of sugarcane. European Journal of Experimental Biology 5(3):13-19.

Toharisman, A., 2007. Kinerja Industri Gula Indonesia, Pusat Penelitian dan Pengembangan Gula Indonesia (P3GI), Pasuruan.

Veresoglou, S.D. Caruso T., & Rillig M.C., 2013. Modelling the environmental and soil factors that shape the niches of two common arbuscular mycorrhizal fungal families. Plant Soil, 368:507-518.

Vieira, L C., Silva D.K.A, Escobar I.E.C, Silva J.M., Moura I.A., Oehl F. & Silva G.A., 2020.Changes in an Arbuscular Mycorrhizal Fungi Community Along an Environmental Gradient. Plants 9 (52):1-16.

Walker C., Mize C.W. & Mcnabb Jr. H.S., 1982. Population of endogonaceus at two location in Central Iowa. Canadian J. of Bot 60: 2518-2529.

Wardhika, C.M., B. Hadisutrisno, & J. Widada, 2015. Potensi Jamur Mikoriza Arbuskular Unggul dalam Peningkatan Pertumbuhan dan Kesehatan Bibit Tebu (Saccharum officinarum L.). Ilmu Pertanian 18(2):84-91.

Downloads

Published

2021-09-04

Issue

Vol. 9 No. 2 (2021)

Section

Articles

License

COPYRIGHT

PLANTA TROPIKA is committed to its authors to protect and defend their work and their reputation and takes allegations of infringement, plagiarism, ethical disputes, and fraud very seriously. PLANTA TROPIKA is published under the terms of the Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). Authors retain copyright and grant the journal right of first publication (online and print) with the work simultaneously.

LICENSE

1. License to Publish
The non-commercial use of the article will be governed by the Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). The author hereby grants PLANTA TROPIKA an exclusive publishing and distribution license in the manuscript include tables, illustrations or other material submitted for publication as part of the manuscript (the “Article”) in print, electronic and all other media (whether now known or later developed), in any form, in all languages, throughout the world, for the full term of copyright, and the right to license others to do the same, effective when the article is accepted for publication. This license includes the right to enforce the rights granted hereunder against third parties.

2. Author’s Warranties
The author warrants that the article is original, written by stated author/s, has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).

3. User Rights
Under the Creative Commons Attribution-Non Commercial 4.0 International (CC BY-NC 4.0) license, the author(s) and users are free to share (copy and redistribute the material in any medium or format) and adapt (remix, transform, and build upon the material). Users must give appropriate credit, provide a link to the license, and indicate if changes were made.

4. Rights of Authors
 Authors retain the following rights:

  • Copyright, and other proprietary rights relating to the article, such as patent rights,
  • The right to use the substance of the article in future own works, including lectures and books,
  • The right to reproduce the article for own purposes, provided the copies are not offered for sale, and
  • The right to self-archive the article.

5. Co-Authorship
If the article was prepared jointly with other authors, the signatory of this form warrants that he/she has been authorized by all co-authors to sign this agreement on their behalf, and agrees to inform his/her co-authors of the terms of this agreement.

6. Royalties
This agreement entitles the author to no royalties or other fees. To such extent as legally permissible, the author waives his or her right to collect royalties relative to the article in respect of any use of the article by PLANTA TROPIKA or its sublicensee.

7. Miscellaneous
PLANTA TROPIKA will publish the article (or have it published) in the Journal if the article’s editorial process is successfully completed and PLANTA TROPIKA or its sublicensee has become obligated to have the article published. PLANTA TROPIKA may conform the article to a style of punctuation, spelling, capitalization, and usage that it deems appropriate.