Acceleration of Echinacea purpurea (L.) Moench Shoot Growth by Benzyl Adenine and Indole Butyric Acid Addition

Nur Rahmawati Wijaya, Heru Sudrajad

Abstract


Echinacea (Echinacea purpurea (L.) Moench) is a medicinal plant known to boost the immune system. Propagation is necessary to increase production. One of the methods of propagation in tissue culture. This research was conducted to understand the most suitable concentration of plant growth regulators. The treatment was given a combination of Benzyl Adenine (BA) and Indole Butyric Acid (IBA) with BA concentration of 1 ppm, 2 ppm, 3 ppm and 4 ppm while the IBA concentration used was 1 ppm and 2 ppm. The next step was subculture by using the combination among IBA 0 ppm, 0.5 ppm, 0.75 ppm and BAP 0 ppm, 0.5 ppm, 0.75 ppm of BAP. The result showed that the most shoots produced by the combination treatment of BA 2 ppm and IBA 1 ppm while the highest shoot and leaf number is best produced in treatment BA 1 ppm and IBA 2 ppm. The largest number of shoots was shown by treatment BA 2 ppm and IBA 1 ppm. This study can be concluded that BA 1 ppm and IBA 2 ppm, and BA 2 ppm and IBA 1 ppm gave the best treatment for shoot growth and control for root induction.


Keywords


Echinacea purpurea; Echinacea; Benzyl Adenine; Indole Butyric Acid; Callus

Full Text:

PDF

References


Abbasi, B. H., Murch, S., & Saxena, P. K. (2007). Echinacea biotechnology : Challenges and opportunities. In Vitro Cell.Dev.Biol.—Plant, 43, 481–492. https://doi.org/10.1007/s11627-007-9057-2

Agustina, L. . (2015). Pengaruh Konsentrasi Zat Pengatur Tumbuh Auksin Golongan NAA dan Waktu Penyiangan terhadap Pertumbuhan dan Hasil Kacang Hijau (Vigna radiata L.). Agroswagati, 13(2), 364–374.

Alamgir, M., & Uddin, S. J. (2010). Recent Advances on The Ethnomedicinal Plants as Immunomodulatory Agents. Ethnomedicine: A Source of Complementary Therapeutics, 37/661(2), 227–244.

Darmawati, I. A. P., Dwiyani, R., & Hestin, D. A. N. (2013). Induksi Kalus dengan 2,4-D pada Mikropropagasi Tanaman Stroberi ( Fragraria x ananassa Duch cv . Rosalinda ). Agrotrop, 3(2), 21–26.

Dinarti, D., Sayekti, U., & Alitalia, Y. (2010). Kultur Jaringan Kantong Semar (Nepenthes mirabilis). J.Hort. Indonesia, 1(2), 59–65.

Hariadi, H., Yusnita, Riniarti, M., & Hapsoro, D. (2019). Pengaruh Arang Aktif, Benziladenin, dan Kinetin terhadap Pertumbuhan Tunas Jati Solomon (Tectona grandis Linn. f) In Vitro. Jurnal Biologi Eksperimen Dan Keanekaragaman Hayati, 5(2), 21–30.

Hidayat. (2007). Induksi Pertumbuhan Eksplan Endosperm Ulin dengan IAA dan Kinetine. Agritrop, 26(4), 147–152.

Hudson, J. B. (2012). Applications of the Phytomedicine Echinacea purpurea (Purple Coneflower) in Infectious Diseases. Journal of Biomedicine and Biotechnology, 2012, 1–16. https://doi.org/10.1155/2012/769896

Ikeuchi, M., Sugimoto, K., & Iwase, A. (2013). Plant Callus : Mechanisms of Induction and Repression. The Plant Cell, 25, 3159–3173. https://doi.org/10.1105/tpc.113.116053

Indah, P. N., & Ermavitalini, D. (2013). Induksi Kalus Daun Nyamplung (Calophyllum inophyllum Linn.) pada Beberapa Kombinasi Konsentrasi 6-Benzylaminopurine (BAP) dan 2,4-Dichlorophenoxyacetic Acid (2,4-D). Jurnal Sains dan Seni Pomits, 2(1), 1–6.

Kumar, K. ., & Ramaiah, S. (2011). Pharmacological Importance of Echinacea purpurea. International Journal of Pharma and Bio Sciences, 2(2), 304–414.

Lestari, E. G. (2011). Peranan Zat Pengatur Tumbuh dalam Perbanyakan Tanaman melalui Kultur Jaringan. Agrobiogen, 7(1), 63–68.

Mahadi, I., Wulandari, S., & Trisnawati, D. (2013). Pengaruh Pemberian NAA dan Kinetin Terhadap Pertumbuhan Eksplan Buah Naga (Hylocereus costaricensis) melalui Teknik Kultur Jaringan secara In Vitro. Jurnal Biogenesis, 9(2), 14–20. http://www.albayan.ae

Manayi, A., Vazirian, M., & Saeidnia, S. (2015). Echinacea purpurea : Pharmacology, phytochemistry, and analysis methods. Pharmacognosy Reviews, 9(17), 63–73. https://doi.org/10.4103/0973-7847.156353

Mechanda, S. M., Baum, B. R., & Johnson, D. A. (2003). Direct Shoot Regeneration from Leaf Segments of Mature Plants of Echinacea purpurea ( L .) Moench. In Vitro Cell. Dev. Biol.—Plant, (October), 505–509. https://doi.org/10.1079/IVP2003461

Menkes. Peraturan Pemerintah Nomor 88 Tahun 2013 (2013).

Moltó, J., Valle, M., Miranda, C., Cedeño, S., Negredo, E., & Clotet, B. (2012). Herb-Drug Interaction between Echinacea purpurea and Etravirine in HIV-Infected Patiens. Antimicrobial Agents and Chemotherapy, 56(10), 5328–5331. https://doi.org/10.1128/AAC.01205-12

Niedz, R. P., & Evens, T. J. (2011). The Effects of Benzil Adenine and Meta-topolin on In Vitro Shoot Regeneration of Sweet Orange. ARPN Journal of Agricultural and Biological Science, 6(3), 64–73.

Putra, R. R., & Shofi, M. (2015). Pengaruh Hormon Napthalen Acetic Acid terhadap Inisiasi Akar Tanaman Kangkung Air ( Ipomoea aquatica Forssk .) Jurnal Wiyata, 2(2), 108–113.

Raharjo. (2005). Peluang Pembudidayaan Tanaman Echinacea (Echinacea purpurea) di Indonesia. Jurnal Prespektif, 4(1), 1–10.

Rahayu, B., Solichatun, & Endang, A. (2003). Pembentukan dan Pertumbuhan Kalus serta Kandungan Flavonoid Kultur Kalus Acalipha indica. Biofarmasi, 1(1), 1–6.

Rahmi, I., Suliansyah, I., & Bustamam, T. (2010). Pengaruh Pemberian Beberapa Konsentrasi BAP dan NAA terhadap Multiplikasi Tunas Pucukjeruk Kanci ( Citrus Sp ) Secara In Vitro. Jerami, 3(3), 210–219.

Rosmaina, & Aryani, D. (2015). Optimasi NAA dan BAP terhadap Pertumbuhan dan Perkembangan Tunas Mikro Tanaman Kantong Semar (Nepenthes mirabilis) Secara In Vitro. Jurnal Agroteknologi, 5(2), 29–36. https://doi.org/10.24014/ja.v5i2.1352

Rout, G. R., Mohapatra, A., & Jain, S. M. (2006). Tissue Culture of Ornamental Pot Plant : A Critical Review on Present Scenario and Future Prospects. Biotechnology Advances 24, 24, 531–560. https://doi.org/10.1016/j.biotechadv.2006.05.001

Salim, H., Myrna, N., & Yulia, A. (2010). Pertumbuhan Bibit Manggis Asal Seedling(Garcinia Mangostana L.) Pada Berbagai Konsentrasi IBA. Jurnal Penelitian Universitas Jambi Seri Sains., 12(2007), 19–24.

Santosa, S., & Soekendarsi, E. (2018). Utilization of Rice and Coconut Water Waste to Accelerate The Growth of Syzygium myrtifolium ( Roxb ) Walp Seedlings on Sediment Media. Academic Research International, 9(December), 1–5.

Sidhu, Y. (2010). In Vitro Micropropagation of Medicinal Plants by Tissue Culture. The Playmouth Student Scientist, 4(1), 432–449.

Sudrajad, H., & Saryanto. (2011). Pengaruh Penambahan Sitokinin pada Senyawa Flavonoid Kalus ( Echinacea purpurea L ). Prosiding Seminar Nasional "Peranan dan Kontribusi Herbal dalam Terapi Penyakit Degeneratif .pp. 102–108.

Sukmadjaja, D., & Mulyana, A. (2011). Regenerasi dan Pertumbuhan Beberapa Varietas Tebu ( Saccharum officinarum L .) secara In Vitro. Jurnal AgroBiogen, 7(2), 106–118.

Sulichantini, E. D. (2016). Pengaruh Konsentrasi Zat Pengatur Tumbuh Terhadap Regenerasi Bawang Putih ( Allium sativum L ). Jurnal Agrifor, 16(1), 29–36.

Tajuddin, T., Karyanti, Sukarnih, T., & Haska, N. (2015). The Combination of Growth Hormones Increased The In Vitro Shoots Multiplication on Sago Palm ( Metroxylon Sagu Rottb.). Jurnal Bioteknologi dan Biosains Indonesia, 2(1), 27–33.

Widyastoety, D. (2014). Pengaruh Auksin dan Sitokinin terhadap Pertumbuhan Planlet Anggrek Mokara. Jurnal Hortikultura, 24(3), 230–238.

Yuliawan, W. (2019). Pertumbuhan Beberapa Bentuk Potongan Pangkal Setek Tanaman Mawar (Rosa sp.) Akibat Cara Aplikasi Zat Pengatur Tumbuh Root-Up. Jurnal Ilmiah Pertanian Paspalum, 7(1), 42–47.

Yusnita, Wahyuningsih, T., Sulistiana, P., & Hapsoro, D. (2013). Perbanyakan In Vitro Sansevieria trifasciata ‘ Lorentii ’: Regenerasi Tunas, Pengakaran, dan Aklimatisasi Planlet In Vitro Propagation of Sansevieria trifasciata ‘Lorentii’: Shoot Regeneration, Rooting, and Plantlet Acclimatization. J. Agron. Indonesia, 41(1), 70–76.




DOI: https://doi.org/10.18196/pt.2019.101.117-124

Article Metrics

Abstract view : 18 times
PDF - 11 times

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 Nur Rahmawati Wijaya

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

 

 

 


 


 







 

Alamat Redaksi
Redaksi Planta Tropika: Jurnal Agrosains (Journal of Agro Science)
Universitas Muhammadiyah Yogyakarta
Jl. Ring Road Selatan, Tamantirto, Kasihan, Bantul
Telp (0274) 387646 psw 224.
Email: plantatropika@umy.ac.id
E-ISSN: 2528-7079
p-ISSN: 0216-499X

 

Creative Commons License
Planta Tropika: Jurnal Agro Sains (Journal of Agroscience) is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.