Detection and Identification of Polymorphism in Mutant Strawberry (Fragaria spp.) Plants Based on Cleaved Amplified Polymorphic Sequences Molecular Markers

Ganies Riza Aristya, Melin Ayundai, Fauzana Putri, Ani Widiastuti, Rina Sri Kasiamdari

Abstract


Strawberry (Fragaria spp.) has a high economic value and various benefits, but the production of strawberry plants in Indonesia is still low in terms of both quantity and quality. Strawberry plant breeding can be done in various techniques, one of which is polyploidization. Polyploidization by an induction of colchicine at various concentrations in strawberry crops cv. California and Festival resulted in superior phenotype characteristics. To prove the existence of a change in ploidy in strawberry, then research at the molecular level needs to be done. The purposes of this study were to find out changes in ploidy of strawberry plants using CAPS molecular markers and to detect the polymorphism in strawberry plants quickly. The samples used were young leaves. Main procedure was the cutting of the amplified DNA using restriction enzymes of TaqI and HaeIII. The results showed that CAPS molecular markers were capable of detecting polymorphism quickly and efficiently in strawberry plants. Specific bands among strawberry plants having undergone polyploidization and those not having undergone polyploidization can be seen on the differences in monomorphic or polymorphic bands between the control plants and treated plants. 


Keywords


Cleaved Amplified Polymorphic Sequence; colchicine; strawberries

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References


Alyza, Rezika. (2015). Karakterisasi Kromosom Stroberi Festival (Fragaria x ananassa D. cv. Festival) Hasil Poliploidisasi. Naskah Skripsi. Fakultas Biologi Universitas Gadjah Mada. Yogyakarta

Budiman, S. dan D. Saraswati. (2005). Berkebun Stroberi Secara Komersial. Penebar Swadaya. Jakarta

David, R. J. (1989). Biochemistry, International Edition. ISBN 0-89278-405-9

Doyle, J. J and Doyle Doyle, J. L. (1990). Isolation of plant DNA from fresh tissue. Focus. 12: 13-15

Fatchiyah., Arumingtyas. E. L., Widyarti. S., dan Rahayu, S. (2011). Biologi Molekular Prinsip Dasar Analisis. Erlangga. Jakarta

Hartl, D. L., and E. J. Jones. (1998). Genetics: Principles and Analysis. Jones and Bartlett Publishers. Sudburry, Massachusetts

Khoiroh, R. (2015). Karakterisasi Kromosom Stroberi (Fragaria vesca L. subsp. Californica Cham. & Schltdl. cv. Californica) Hasil Poliploidisasi. Naskah Skripsi. Fakultas Biologi Universitas Gadjah Mada. Yogyakarta

Konieczny A, Ausubel FM. (1993). A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. Plant J. Aug. 4(2):403-10

Kunihisa, M., Fukino, N., Matsumoto, S., (2005). CAPS markers improved by cluster-specific amplification for identification of octoploid strawberry (Fragaria×ananassa Duch.) cultivars, and their disomic inheritance. Theor. Appl. Genet. 110: 1410–1418

Matsumoto, A. and Y. Tsumura. (2004). Evaluation of cleaved amplified polymorphic sequence markers. Theoretical and Applied Genetics. 110: 80–91

Sambrook , J., and D.W.Russel. (2001). Molecular Cloning : A Laboratory manual. 3rd ed. Cold Spring Harbor : Cold Spring Harbor Laboratory Press. ISBN 0-87969-309-6

Sato, S. (1978). A single cleavage of Simian virus 40 (SV40) DNA by a site specific endonuclease from Thermus aquaticus, Taq I. J. Biochem. Tokyo. 83 (2): 633–5




DOI: https://doi.org/10.18196/pt.2020.109.15-20

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Creative Commons License
Planta Tropika: Jurnal Agrosains (Journal of Agro Science) is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.