Microscopic Characterization of Fusarium sp. Associated with Yellow Disease of Pepper (Piper nigrum L.) in South Bangka Regency
DOI:
https://doi.org/10.18196/pt.v9i1.7753Keywords:
Fusarium sp., yellow disease, Piper nigrum Linn.Abstract
Pepper production has decreased recently, especially due to yellow diseases of Fusarium sp. Thus, this research aimed to isolate and characterize Fusarium sp. from soil and root of healthy and diseased pepper plants. The sampling technique used was purposive sampling. Soil and root pepper samples were taken from lands in Payung and Ranggung Village, Payung District, South Bangka Regency. There were 3 varieties of pepper plant used, including Petaling 1, Nyelungkup, and Merapin Daun Kecil. The characterization of Fusarium sp. isolate included macroscopic and microscopic observation. Macroscopic observation included colony color, colony base color, and growth rate/colony diameter size (cm), while microscopic observation included hyphae structure, and the shape and size of microconidia, macroconidia, chlamydospore, and conidiophore. The research found 66 isolates of Fusarium genus based on the colony color. Most of the isolates were white or purple and red. Colony color of Fusarium sp. showed white color, which then turned to orange color. All isolates showed septate hyphae. Isolates with macroconidia 3-4 septate and micronidia 0-1 septate showed the character of Fusarium oxysporum, while isolates with macroconidia 3-5 septate and microconidia 0-2 septate showed the character of Fusarium solani.
References
Barnett, H. & Hunter, B. (2006). Illustrated Genera of Imperfect Fungi (4th edition). St. Paul, Minnesota (USA): Amer Phytopathological Society.
BPS Babel [Badan Pusat Statistik Bangka Belitung]. (2018). Provinsi Kepulauan Bangka Belitung dalam Angka 2017. Pangkalpinang: Badan Pusat Statistik Provinsi Kepulauan Bangka Belitung.
Daras, U., & Pranowo, D. (2009). Kondisi Kritis Lada Putih Bangka Belitung dan Alternatif Pemulihannya. Jurnal Litbang Pertanian, 28(1), 1–6.
Ditjenbun [Direktorat Jenderal Perkebunan]. (2018). Statistik Perkebunan Indonesia Komoditas Lada 2015-2017. Jakarta: Direktorat Jenderal Perkebunan.
Fadilah, L. (2016). Eksplorasi Cendawan Antagonis Terhadap Septbasidium pseudopedicellatum Burt. Penyebab Penyakit Hawar Beludru pada Lada. [Skripsi]. Bogor: Institut Pertanian Bogor.
Harni, R., & Munif, A. (2012). Pemanfaatan Agens Hayati Endofit untuk Mengendalikan Penyakit Kuning pada Tanaman Lada. Buletin RISTRI, 3(3), 201–206.
Kardinan, A., Laba, I. W., & Rismayani. (2018). Peningkatan Daya Saing Lada (Piper nigrum L.) Melalui Budidaya Organik. Perspektif, 17(1), 26-39.
Kim, H. G., Han, E. H., Jang, W. S., Choi, J. H., Khanal, T., Park, B. H., … Jeong, H. G. (2012). Piperine inhibits PMA-induced cyclooxygenase-2 expression through downregulating NF-κB, C/EBP and AP-1 signaling pathways in murine macrophages. Food and Chemical Toxicology, 50(7), 2342–2348.
Leslie, J. & Summerell, B. (2006). The Fusarium Laboratory Manual. Victoria: Blackwell Publishing.
Mukarlina, Khotimah, S., & Rianti, D. A. N. R. (2010). Layu pada Tanaman Cabai (Capsicum annum) Secara In Vitro. Jurnal Fitomedika, 7(2), 80–85.
Munif, A., & Kristiana. (2012). Hubungan Bakteri Endofit dan Nematoda Parasit Bangka Belitung. Buletin RISTRI, 3(1), 71–78.
Munif, A., & Sulistiawati, I. (2014). Pengelolaan Penyakit Kuning pada Tanaman Lada oleh Petani di Wilayah Bangka. Jurnal Fitopatologi Indonesia, 10(1), 8–16.
Prabhu, G. R. D., Kiran, C. R., Sundaresan, A., Mony, R. S., & Venugopalan, V. V. (2015). Process development studies for recovery of bio active isolates from spent black pepper generated from ayurvedic industry. Industrial Crops and Products, 66, 144–149.
Ropalia, R. (2017). Potency of Bamboo Root Endophytes as Biocontrol to Fusarium oxysporum Pathogen Cause Yellowing Disease on Pepper Plant. AGROSAINSTEK: Jurnal Ilmu Dan Teknologi Pertanian, 1(2), 80–85.
Sari, W., Wiyono, S., Nurmansyah, A., Munif, A., & Poerwanto, R. (2017). Keanekaragaman dan Patogenisitas Fusarium spp. Asal Beberapa Kultivar Pisang. Jurnal Fitopatologi Indonesia, 13(6), 216–228.
Semangun, H. (2008). Penyakit-penyakit Tanaman Perkebunan di Indonesia. Yogyakarta: Gadjah Mada University Press.
Shahnazi, S., Meon, S., Vadamalai, G., Ahmad, K., & Nejat, N. (2012). Morphological and molecular characterization of Fusarium spp. associated with yellowing disease of black pepper (Piper nigrum L.) in Malaysia. Journal of General Plant Pathology, 78(3), 160–169.
Son, D. J., Kim, S. Y., Han, S. S., Kim, C. W., Kumar, S., Park, B. S., … Park, Y. H. (2012). Piperlongumine inhibits atherosclerotic plaque formation and vascular smooth muscle cell proliferation by suppressing PDGF receptor signaling. Biochemical and Biophysical Research Communications, 427(2), 349–354.
Sudarma, I., Puspawati, N., Suniti, N., Wijaya, I., & Bagus, I. (2015). Utilization of Rhizosphere Fungi to Control Fusarium Oxysporum f.Sp. Capsici In Vitro. International Journal of Biosciences and Biotechnology, 2(2), 83–92.
Suryanti, S., Hadisutrisno, B., Mulyadi, M., & Widada, J. (2017). Interaksi Meloidogyne incognita dan Fusarium solani pada Penyakit Kuning Lada. Jurnal Perlindungan Tanaman Indonesia, 21(2), 127–134.
Suryanti, Hadisutrisno, B., Mulyadi, & Widada, J. (2013). Survey of Distribution Pepper Yellowing Disease and Pathogens Associated. Jurnal Budidaya Pertanian, 9(2), 60–63.
Suryanti, Hadisutrisno, B., Mulyadi, & Widada, J. (2015). Fusarium Identification and Plant Parasitic Nematode Associated With Pepper Yellowing Disease in West Kalimantan. Jurnal Perlindungan Tanaman Indonesia, 19(1), 19–26.
Sutejo, A. M., & Priyatmojo, A. (2008). Identifikasi Morfologi Beberapa Spesies Jamur Fusarium. Jurnal Perlindungan Tanaman Indonesia, 14(1), 7–13.
Suwandi, Mazid, A., & Surahman. (2001). Populasi Bakteri Antagonistik Terhadap Fusarium sp. dari Rizosfer Lada Sehat dan Sakit Kuning. Jurnal Perlindungan Tanaman Indonesia, 7(1), 59–64.
Taufik, M., Khaeruni, A., Wahab, A., & Amiruddin. (2011). Agens hayati dan Arachis pintoi memacu pertumbuhan tanaman lada (Piper nigrum) dan mengurangi kejadian penyakit kuning. Menara Perkebunan, 79(2), 42–48.
Watanabe, T. (2010). Pictorial Atlas of Soil and Seed Fungi Morphologies of Cultured Fungi and Key to Species (3rd Editio). Boca Raton: CRC Press.
Downloads
Published
Issue
Section
License
PLANTA TROPIKA is committed to its authors to protect and defend their work and their reputation and takes allegations of infringement, plagiarism, ethical disputes, and fraud very seriously. PLANTA TROPIKA is published under the terms of the Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). Authors retain copyright and grant the journal right of first publication (online and print) with the work simultaneously.
LICENSE
1. License to Publish
The non-commercial use of the article will be governed by the Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). The author hereby grants PLANTA TROPIKA an exclusive publishing and distribution license in the manuscript include tables, illustrations or other material submitted for publication as part of the manuscript (the “Article”) in print, electronic and all other media (whether now known or later developed), in any form, in all languages, throughout the world, for the full term of copyright, and the right to license others to do the same, effective when the article is accepted for publication. This license includes the right to enforce the rights granted hereunder against third parties.
2. Author’s Warranties
The author warrants that the article is original, written by stated author/s, has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).
3. User Rights
Under the Creative Commons Attribution-Non Commercial 4.0 International (CC BY-NC 4.0) license, the author(s) and users are free to share (copy and redistribute the material in any medium or format) and adapt (remix, transform, and build upon the material). Users must give appropriate credit, provide a link to the license, and indicate if changes were made.
4. Rights of Authors
Authors retain the following rights:
- Copyright, and other proprietary rights relating to the article, such as patent rights,
- The right to use the substance of the article in future own works, including lectures and books,
- The right to reproduce the article for own purposes, provided the copies are not offered for sale, and
- The right to self-archive the article.
5. Co-Authorship
If the article was prepared jointly with other authors, the signatory of this form warrants that he/she has been authorized by all co-authors to sign this agreement on their behalf, and agrees to inform his/her co-authors of the terms of this agreement.
6. Royalties
This agreement entitles the author to no royalties or other fees. To such extent as legally permissible, the author waives his or her right to collect royalties relative to the article in respect of any use of the article by PLANTA TROPIKA or its sublicensee.
7. Miscellaneous
PLANTA TROPIKA will publish the article (or have it published) in the Journal if the article’s editorial process is successfully completed and PLANTA TROPIKA or its sublicensee has become obligated to have the article published. PLANTA TROPIKA may conform the article to a style of punctuation, spelling, capitalization, and usage that it deems appropriate.
