Histopathological Evaluation of Soybean (Glycine max (L.) Merr.) Strains Resistance to Sclerotium rolfsii Disease

Authors

  • Siti Samiyarsih Department of Botany, Faculty of Biology, University of Jenderal Soedirman. Jalan Dr. Suparno No. 63 Purwokerto 53122, Banyumas, Central Java http://orcid.org/0000-0002-6501-8282
  • Chindy Ayu Erlina Department of Botany, Faculty of Biology, University of Jenderal Soedirman. Jalan Dr. Suparno No. 63 Purwokerto 53122, Banyumas, Central Java
  • Juni Safitri Muljowati Department of Microbiology, Faculty of Biology, University of Jenderal Soedirman Jalan Dr. Suparno No. 63 Purwokerto 53122, Banyumas, Central Java http://orcid.org/0000-0003-2011-7700
  • Nur Fitrianto Research and Technology Center for Application of Isotope and Radiation, National Research and Innovation Agency of Indonesia (BRIN); Jl. Lebak Bulus Raya No. 49 Jakarta 12440

DOI:

https://doi.org/10.18196/pt.v10i1.8907

Keywords:

Glycine max, Histopathology, Resistance, Sclerotium rolfsii

Abstract

Sclerotinia infection of stem and leaf of soybean Glycine max (L.) Merr. caused by the fungal pathogen of Sclerotium rolfsii has recently become more important in the Indonesian soybean production area. This study aimed to evaluate the level of resistance and intensity of infection by S. rolfsii in four soybean strains. The research was arranged in a factorial completely randomized design. The observed variables include the anatomy characteristics of leaves and stems of soybean and disease intensity caused by S. rolfsii. The data were analyzed quantitatively with the Analysis of Variance (ANOVA) at 95% and 99% confidence level, followed by the Least Significant Difference Test (Fisher’s LSD) at the level of 5%. Soybean leaves and stem anatomy inoculated by S. rolfsii showed a decrease in the stomatal density, epidermis thickness, and mesophyll thickness as well as a damaged cuticle, damaged stem epidermis, and swollen stem cortex. Four strains inoculated by S. rolfsii showed a higher disease intensity of 40%-80% compared to the resistant cultivar ('Dering') and susceptible cultivar ('Wilis'), showing disease intensity of 20% and 40%, respectively.

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Published

2022-02-28

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Vol. 10 No. 1 (2022)

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