Pyknotic Cell Features of Buccal Mucosal Smear in Various Oral Lesions of Betel Nut Chewers

Authors

  • Maulina Triani Department of Oral Medicine, Dentistry Study Program, Faculty of Medicine, Jenderal Sudirman University
  • Akbar Aulia Hidayat Department of Pediatric Dentistry, Faculty of Dental Medicine, Gadjah Mada University
  • Aris Aji Kurniawan Department of Oral Medicine, Dentistry Study Program, Faculty of Medicine, Jenderal Sudirman University
  • Anindita Laksitasari Department of Oral Medicine, Dentistry Study Program, Faculty of Medicine, Jenderal Sudirman University
  • Aditya Priagung Prakosa Department of Pediatric Dentistry, Faculty of Dental Medicine, Gadjah Mada University

DOI:

https://doi.org/10.18196/di.v11i1.14561

Keywords:

Apoptosis, Betel nut, OSCC, Pyknotic

Abstract

The habit of chewing betel nut is one of the predisposing factors that can trigger the formation of various oral lesions and develop into a malignant condition in the form of oral squamous cell carcinoma (OSCC). This malignancy has a low five-year survival rate as early detection is not widely carried out. Early detection can be conducted by examining pyknotic cells to describe the level of apoptosis. This study aims to examine the pyknotic cell features as a marker of apoptosis in various oral lesions found in betel nut chewers. This study is an analytic survey conducted once with a post-test control group design. The study was conducted on 17 betel nut chewers and 17 healthy people as controls. A buccal mucous smear was collected, and cytological slides were made with Papanicolaou stain. Pyknotic cells were counted using a light microscope and Optilab pro camera. This study found several oral lesions in betel nut chewers, namely exogenous pigmentation, lesions resembling leukoplakia, and lichenoid reaction. Furthermore, the result also showed that the frequency of pyknotic cells in betel nut chewers was lower than in the control group. Non-parametric Kruskal Wallis and post hoc Mann Whitney test showed significant differences in the number of pyknotic cells in various oral lesions with a p-value = 0,001. It can be concluded that the consumption of betel nut affected the level of apoptosis, characterized by a decrease in pyknotic cells and significantly different pyknotic cells depending on the type of oral lesions.

Author Biography

Maulina Triani, Department of Oral Medicine, Dentistry Study Program, Faculty of Medicine, Jenderal Sudirman University

departemen ilmu penyakit mulut

References

Amtha R, Razak IA, Basuki B, Roeslan, BO, Gautama W, Puwanto DJ, Ghani WMN, Zain RB. Tobacco (Kretek) smoking, betel quid chewing and risk of oral cancer in a selected Jakarta population. Asian Pacific Journal of Cancer Prevention. 2014;15(20):8673-8678. https://doi.org/10.7314/APJCP.2014.15.20.8673

Watopa JJ. Betel Nut Chewing Behavior among Adolescents in Papua Province, Indonesia. Human Behavior, Development and Society. 2019;20(1):31-38. https://doi.org/10.35974/isc.v6i1.1416

Chen PH, Mahmood Q, Mariottini GL, Chiang TA, Lee KW. Adverse health effects of betel quid and the risk of oral and pharyngeal cancers. BioMed Research International. 2017;1-25. https://doi.org/10.1155/2017/3904098

Binmadi N, Harere L, Mattar A, et al. Oral lesions associated with smokeless tobacco users in Saudi Arabia: Single center cross-sectional study. Saudi Dental Journal. 2022;34(2):114-120. https://doi.org/10.1016/j.sdentj.2021.12.002

H Khan M, P M Mishu, Touseef Imam S. Current Molecular Concept of Oral Carcinogenesis and Invasion. Med. Today. 2010; 22(1);38-42. https://doi.org/10.3329/medtoday.v22i1.5605

Patil D. Emergence of micronuclei and apoptosis as a potential biomarker of oral carcinogenesis: An updated review. Asian Journal of Oncology. 2018;04(01):011-016. https://doi.org/10.4103/ASJO.ASJO_15_18

Tandelilin RT, Mun TS, Haniastuti T. Assessment of Karyorrhexis Incidence in Exfoliated Buccal Mucosa Epithelial Cells among Fuel Station Employees in Sleman, Special Region of Yogyakarta, Indonesia. Int. J. Exp. Dent. Sci. 2020;9(2):62-69. https://doi.org/10.5005/jp-journals-10029-1216

Dahlan. Statistik Untuk Kedokteran Dan Kesehatan: Deskriptif, Bivariat, Dan Bivariat, Dilengkapi Aplikasi Menggunakan SPSS. 6th ed. Epidemiologi Indonesia; 2014.

Nur Sazwi N, Nalina T, Haji Abdul Rahim Z. Antioxidant and Cytoprotective Activities of Piper Betle, Areca Catechu, Uncaria Gambir and Betel Quid with and without Calcium Hydroxide. BMC Complement Altern Med. 2013;13:351 https://doi.org/10.1186/1472-6882-13-351

Wang TH, Hsia SM, Shih YH, Shieh TM. Association of smoking, alcohol use, and betel quid chewing with epigenetic aberrations in cancers. Int. J. Mol. Sci. 2017;18(6); 1-14. https://doi.org/10.3390/ijms18061210

Hernandez BY, Zhu X, Goodman MT, et al. Betel nut chewing, oral premalignant lesions, and the oral microbiome. PLoS ONE. 2017;12(2):1-19. https://doi.org/10.1371/journal.pone.0172196

Bn N, Najem H, Alva SR, Student PG. Comparison of Cytogenetic Abnormality of Exfoliative Buccal Cells among Smokers and Non-Smokers. IP Arch Cytol Histopathol Res, 2017;2(1):1-4.

Farooq I, Bugshan A. Oral squamous cell carcinoma: Metastasis, potentially associated malignant disorders, etiology and recent advancements in diagnosis. F1000 Res.2020;9:229. https://doi.org/10.12688/f1000research.22941.1

Yanduri S, Kumar VB, Suma S, Madhura MG. Lichenoid Features and Fibrosis: Coexistence in Quid-induced Oral Lesions. J. Contemp. Dent. 2015;16(5):389-393. https://doi.org/10.5005/jp-journals-10024-1695

Karthik R, Mohan N. Prevalence of oral mucosal lesions among dental patients with mixed habits in Salem district - A study. J. Pharm. Bioallied Sci. 2017;9(5):55-67. https://doi.org/10.4103/jpbs.JPBS_86_17

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Published

2022-05-28

Issue

Vol. 11 No. 1 (2022): May

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Articles

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