The Effectiveness of α-Mangostin in Reducing the Streptococcus Mutans Biofilm Thickness

Rahmawati Sri Praptiningsih; Arlina Nurhapsari; Rifqi Wahyu Febrian

doi:10.18196/di.v11i2.15809

The Effectiveness of α-Mangostin in Reducing the Streptococcus Mutans Biofilm Thickness

Rahmawati Sri Praptiningsih, Arlina Nurhapsari, Rifqi Wahyu Febrian

Abstract

Dental caries occurs due to the demineralization of tooth structure caused by microorganisms in colonies called biofilms. One of the microorganisms involved in dental caries is Streptococcus mutans. Oral mouthwash, in addition to mechanical cleaning, is known to prevent the growth of oral microorganisms. Mangosteen is known as an anti-cancer ingredient with high anti-bacterial properties. This study aims to identify the effectiveness of mangosteen skin extract (Garcinia mangostana L.) in decreasing Streptococcus mutans biofilm. The study is experimental research with a post-test control group design. The research sample was divided into five groups; 3 treatment groups and 2 control groups. The biofilm thickness test was carried out with OD (Optical density) with a wavelength of about 620 nm using an ELISA reader. Kruskal Wallis analysis was employed as a non-parametric statistical test analysis. Statistical Kruskal Wallis indicated significant differences in the thickness of 5 test groups of Streptococcus mutans. The lowest average yield of biofilm thickness was in the α-mangosteen group at 12.5 g/ml. Conclusion: α-mangosteen in mangosteen skin extract effectively reduced the thickness of Streptococcus mutans biofilm.

Keywords

α-mangostin; biofilm thickness; Streptococcus mutans

Full Text:

PDF

References

RISKESDAS. Riset Kesehatan Dasar Tahun 2018 [Internet]. Badan Penelitian dan Pengembangan Kesehatan. 2018. p. 198. Available from: http://labdata.litbang.kemkes.go.id/images/download/laporan/RKD/2018/Laporan_Nasional_RKD2018_FINAL.pdf

Sibarani M. Dental Caries: Etiology, Clinical Characteristics, and Management. Maj Kedokt UKI. 2014;30(1):14-22.

Komansilan JG, Mintjelungan CN, Waworuntu O. Daya Hambat Ekstrak Kulit Manggis (Garcinia Mangostana L.) Terhadap Streptococcus mutans. e-GIGI. 2015;3(2).

https://doi.org/10.35790/eg.3.2.2015.8824

Gunawan PN, Supit A, Manado SR. Uji Efektif Anti Bakteri Esktrak Bunga Cengkeh. 2014;2.

Astuti SI, Arso SP, Wigati PA. Aktivitas Pembentukan Biofilm Streptococcus Mutans dan Candida Albicans Setelah Dipapar Dengan Cigarette Smoke Condensate dan Minuman Probiotik. Anal Standar Pelayanan Minimal Pada Instal Rawat Jalan di RSUD Kota Semarang. 2015;3(Februari):103-11.

Hamsar A, Ramadhan ES. Penggunaan Chlorhexidine Kumur dalam Perbaikan Indeks Kebersihan Gigi Pegawai Poltekkes Kemenkes RI Medan. J Kesehat Gigi. 2019;6(2):99-103.

https://doi.org/10.31983/jkg.v6i2.5482

Dwipriastuti D, Putranto RR, Anggarani W. Perbedaan Efektivitas Chlorhexidine Glukonat 0,2% Dengan Teh Hijau (Camellia Sinensis) Terhadap Jumlah Porphyromonas Gingivalis. ODONTO Dent J. 2017;4:50-4.

https://doi.org/10.30659/odj.4.1.50-54

Al-Eraky MM, Mohamed N, Kamel F, Al-Qahtani A, Madini MA, Hussin A, et al. Chlorhexidine - An Insight. Nazam. Int J Adv Res [Internet]. 2016;4(6):625-34. Available from: http://www.journalijar.com

Darmawansyih. Khasiat Buah Manggis untuk Kehidupan. J Al Hikmah. 2014;XV:60-8.

Idawati S, Hakim A, Andayani Y. Isola α - Mangostin dari Kulit Buah Manggis ( Garcinia mangostana L.) dan Uji Aktivitas Antibakteri terhadap Bacilus cereus. 2018;118-22.

Yanura A. Potensi α-Mangostin dari Ekstrak Perikarp Manggis(Garcinia mangostana L.) dalam Menghambat SekresiProtein Culture FiltrateProtein (CFP)-10 pada Mycobacterium tuberculosis H37Rv. Pharm J Indones. 2016;2(1):12-7.

https://doi.org/10.21776/ub.pji.2016.002.01.3

Syaravina CB, Amalina R, Hadianto E. Pengaruh Ekstrak Daun Beluntas (Pluchea indica (L.) Less) 25% Terhadap Biofilm Streptococcus Mutans - in vitro. ODONTO Dent J. 2018;5(1):28.

https://doi.org/10.30659/odj.5.1.28-33

Wangi RPL, Suswati E, Wisudanti DD. Aktivitas Ekstrak Metanol Bawang Putih ( Allium sativum ) sebagai Penghambat Pembentukan Biofilm pada Pseudomonas aeruginosa ( The Activity of Methanolic Extract of Garlic ( Allium sativum ) in Inhibiting Growth of Biofilm in Pseudomonas aeruginosa ). J Pustaka Kesehat. 2017;5(3):537-43.

Maghfirah F, Saputri D, Basri. Aktivitas pembentukan biofilm Streptococcus mutans dan Candida albicans setelah dipapar dengan cigarette smoke condensate dan minuman probiotik. J Caninus Dent. 2017;2(1):12-9.

Nguyen PTM, Falsetta ML, Hwang G, Gonzalez-Begne M, Koo H. Α-Mangostin Disrupts the Development of Streptococcus Mutans Biofilms and Facilitates Its Mechanical Removal. PLoS One. 2014;9(10).

https://doi.org/10.1371/journal.pone.0111312

Ahyar Afiana; Rakhmawatie, Maya Dian FWR. in Vitro Effect of Alfa Mangostin on Multiresistant Uropathogenic Escherichia Coli. Sains Med J Kedokt dan Kesehat [Internet]. 2017;8(Vol 8, No 1 (2017): January-June 2017):20-7. http://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/1002

https://doi.org/10.26532/sainsmed.v8i1.1002

Phuong NTM, Van Quang N, Mai TT, Anh NV, Kuhakarn C, Reutrakul V, et al. Antibiofilm activity of α-mangostin extracted from Garcinia mangostana L. against Staphylococcus aureus. Asian Pac J Trop Med [Internet]. 2017;10(12):1154-60. https://doi.org/10.1016/j.apjtm.2017.10.022

https://doi.org/10.1016/j.apjtm.2017.10.022

Tamura H. A Novel Herbal Formulation versus Chlorhexidine Mouthwash in Efficacy against Oral Microflora. 2018;8(831):34-7.

Sinaredi BR, Pradopo S, Wibowo TB. Daya Antibakteri Obat Kumur Chlorhexidine, Povidone Iodine, Fluoride Suplementasi Zinc Terhadap Streptococcus mutans dan Porphyromonas gingivalis. Maj Kedokt Gigi. 2014;47(4):211.

https://doi.org/10.20473/j.djmkg.v47.i4.p211-214

DOI: https://doi.org/10.18196/di.v11i2.15809