Combination of Channa striata Extract Gel and HBOT on Trabeculae Area during Orthodontic Treatment

Authors

  • Bunga Fauzia Department of Dental Materials, Faculty of Dentistry, Universitas Hang Tuah, Jl. Arief Rahman Hakim No. 150, Surabaya, 60111, Indonesia https://orcid.org/0000-0002-4513-856X
  • Bambang Sucahyo Department of Orthodontics, Faculty of Dentistry, Universitas Hang Tuah, Jl. Arief Rahman Hakim No. 150, Surabaya, 60111, Indonesia
  • Pambudi Rahardjo Department of Orthodontics, Faculty of Dentistry, Universitas Hang Tuah, Jl. Arief Rahman Hakim No. 150, Surabaya, 60111, Indonesia
  • Arya Brahmanta Department of Orthodontics, Faculty of Dentistry, Universitas Hang Tuah, Jl. Arief Rahman Hakim No. 150, Surabaya, 60111, Indonesia

DOI:

https://doi.org/10.18196/di.v14i1.24691

Keywords:

Orthodontic tooth movement, hyperbaric oxygen therapy, Channa striata, trabecular bone

Abstract

Orthodontic pressure applied to the teeth causes stress and inflammation in the periodontal tissues. Channa striata extract gel and hyperbaric oxygen therapy (HBOT) accelerate the healing process of periodontal tissues under stress due to orthodontic pressure by stimulating osteoblasts to form bone trabeculae in tension areas on bone remodeling. To determine the effect of the combination of Channa striata extract gel and HBOT on the trabecular bone area during orthodontic tooth movement in the tension area. 30 male Cavia cobaya were randomly divided into 5 groups (n=6): negative control (K-) without orthodontic tooth movement (OTM), positive control (K+) with OTM, treatment (P1) with OTM and Channa striata extract gel, treatment (P2) with OTM and HBOT, and treatment (P3) with OTM and combination of Channa striata gel extract and HBOT. Rubber separators were attached to the maxillary central incisors. HBOT 2.4 ATA was administered for 7 days from day 17 to day 23, and Channa striata gel was administered for 14 days from day 10 to day 23. The trabecular bone area was examined using HE staining, and results were calculated using Image Raster. Data were evaluated using the One-Way ANOVA test and LSD (p<0.05). The mean trabecular bone area was the largest in the P3 group (1,126,649.83+90,817.17). Anova and LSD analysis showed that the most significant difference was found in group P2 (p<0.05). The combination of HBOT and Channa striata gel extract affects the trabecular bone area in the tension area during OTM, and the most effective therapy is HBOT

Author Biographies

Bunga Fauzia, Department of Dental Materials, Faculty of Dentistry, Universitas Hang Tuah, Jl. Arief Rahman Hakim No. 150, Surabaya, 60111, Indonesia

Department of Dental Materials, Faculty of Dentistry

Bambang Sucahyo, Department of Orthodontics, Faculty of Dentistry, Universitas Hang Tuah, Jl. Arief Rahman Hakim No. 150, Surabaya, 60111, Indonesia

Department of Orthodontics, Faculty of Dentistry, Universitas Hang Tuah

Pambudi Rahardjo, Department of Orthodontics, Faculty of Dentistry, Universitas Hang Tuah, Jl. Arief Rahman Hakim No. 150, Surabaya, 60111, Indonesia

Department of Orthodontics, Faculty of Dentistry, Universitas Hang Tuah

Arya Brahmanta, Department of Orthodontics, Faculty of Dentistry, Universitas Hang Tuah, Jl. Arief Rahman Hakim No. 150, Surabaya, 60111, Indonesia

Department of Orthodontics, Faculty of Dentistry, Hang Tuah University

References

Maulana H, Hikmah N. The Role of Periodontal Ligament Remodeling on Orthodontic Tooth Movement. 2023;03(11):2636–40. Available from: https://doi.org/10.47191/ijmscrs/v3

Nakai Y, Praneetpong N, Ono W, Ono N. Mechanisms of Osteoclastogenesis in Orthodontic Tooth Movement and Orthodontically Induced Tooth Root Resorption. J Bone Metab. 2023;30(4):297–310. https://pubmed.ncbi.nlm.nih.gov/38073263/

Moga RA, Olteanu CD, Delean AG. Trabecular Bone Component Assessment under Orthodontic Loads and Movements during Periodontal Breakdown-A Finite Elements Analysis. Dent J. 2024 Jun;12(6). https://pubmed.ncbi.nlm.nih.gov/38920891/

Oftadeh R, Perez-Viloria M, Villa-Camacho JC, Vaziri A, Nazarian A. Biomechanics and Mechanobiology of Trabecular Bone: A Review. J Biomech Eng. 2015;137(1):1–15. https://pubmed.ncbi.nlm.nih.gov/25412137/

Šromová V, Sobola D, Kaspar P. A Brief Review of Bone Cell Function and Importance. Cells. 2023;12(21). https://pubmed.ncbi.nlm.nih.gov/37947654/

Rizk M, Niederau C, Florea A, Kiessling F, Morgenroth A, Mottaghy FM, et al. Periodontal ligament and alveolar bone remodeling during long orthodontic tooth movement analyzed by a novel user-independent 3D-methodology. Sci Rep. 2023 Nov;13(1):19919. https://pubmed.ncbi.nlm.nih.gov/37964111/

Fitrianti E, Zulkarnain Z, Nurmayanti N. Efektivitas albumin ekstrak ikan gabus (Channa striata) terhadap penyembuhan luka pascaoperasi pada kucing domestik di UPTD Puskeswan Makassar. Filogeni J Mhs Biol [Internet]. 2023;3(2):79–84. Available from: https://journal.uin-alauddin.ac.id/index.php/filogeni/article/view/34747

Siswanto A, Dewi N, Hayatie L. Effect of haruan (Channa striata) extract on fibroblast cells count in wound healing. J Dentomaxillofacial Sci. 2016;1(2):234. https://jdmfs.org/index.php/jdmfs/article/view/3

Yasin JJ, Brahmanta A, Pargaputri AF. Inovasi Terapi Oksigen Hiperbarik Dan Stichopus hermanii Terhadap Jumlah Makrofag Pada Ligamen Periodontal Antara Daerah Tekanan Dan Tarikan Selama Pergerakan Gigi Ortodonti. Denta. 2019;13(1).

Sandana IKI, Velisia J, Yunior A, Brahmanta A, Prameswari N. Potensi gel Stichopus hermanii dan Hyperbaric Oxygen Therapy untuk mempercepat perawatan ortodonti. Jurnal Kedokteran Gigi Universitas Padjadjaran. 2017;29(3). https://jurnal.unpad.ac.id/jkg/article/view/15951/0

Fadhila FN, Soewondo IK, Nafi’ah N. Efektivitas Pemberian Ekstrak Ikan Haruan (Channa Striata) Terhadap Jumlah Neutrofil Pada Proses Penyembuhan Ulkus Traumatikus Rattus Novergicus Strain Wistar. Dent J Kedokt Gigi. 2018;12(2):89–97.

Sunarno S, Damayanti R, Devi A, Fikri M, Pratiwi F, Ayu L. Aplikasi biomaterial aktif dari daging ikan gabus (Channa striata) untuk penyembuhan luka pascaoperasi pada hewan model tikus Wistar. J Biol Trop. 2018;1(2):13. https://ejournal2.undip.ac.id/index.php/jbt/article/view/3768

Gokce S, Bengi AO, Akin E, Karacay S, Sagdic D, Kurkcu M, et al. Effects of hyperbaric oxygen during experimental tooth movement. Angle Orthod. 2008 Mar;78(2):304–8. https://pubmed.ncbi.nlm.nih.gov/18251603/

Maltha JC, Kuijpers-Jagtman AM. Mechanobiology of orthodontic tooth movement: An update. J World Fed Orthod [Internet]. 2023;12(4):156–60. Available from: https://www.sciencedirect.com/science/article/pii/S2212443823000413

Fadila PN. Biomekanika Pergerakan Gigi Dalam Percepatan Perawatan Ortodonti. J Ilmu Kedokt Gigi. 2020;1:45–58.

Kuten Pella O, Hornyák I, Horváthy D, Fodor E, Nehrer S, Lacza Z. Albumin as a Biomaterial and Therapeutic Agent in Regenerative Medicine. Int J Mol Sci. 2022;23(18). https://pubmed.ncbi.nlm.nih.gov/36142472/

Hanafiah OA, Satria D, Madhy ZA, Wardhani NZ, H. G. AA, Fajar TH, et al. Effectiveness of Cork Fish Extract Gel on Fibroblast Proliferation in Socket Wound After Tooth Extraction. Int J Appl Pharm. 2024;16(2):34–8. https://journals.innovareacademics.in/index.php/ijap/article/view/51589

Tobeiha M, Moghadasian MH, Amin N, Jafarnejad S. RANKL/RANK/OPG Pathway: A Mechanism Involved in Exercise-Induced Bone Remodeling. Biomed Res Int. 2020;2020. https://pmc.ncbi.nlm.nih.gov/articles/PMC7053481/

Kang TS, Gorti GK, Quan SY, Ho M, Koch RJ. Effect of hyperbaric oxygen on the growth factor profile of fibroblasts. Arch Facial Plast Surg. 2004;6(1):31–5. https://pubmed.ncbi.nlm.nih.gov/14732642/

Ramadhani FZ, Brahmanta A, Rahardjo P. Perbedaan Jumlah Osteoblas Pada Pergerakan Gigi Ortodonti yang Diberi Terapi Oksigen Hiperbarik Selama 7 dan 10 Hari. Dent J Kedokt Gigi FKG-UHT [Internet]. 2014;8(10):86–98. Available from: http://dspace.hangtuah.ac.id:8080/xmlui/handle/dx/1337%0Ahttp://dspace.hangtuah.ac.id:8080/xmlui/bitstream/handle/dx/1337/Jurnal Nasional 6.pdf?sequence=1

Forestryana D, Hayati A, Putri AN. Formulation and Evaluation of Natural Gel Containing Ethanolic Extract of Pandanus amaryllifolius R. Using Various Gelling Agents. Borneo J Pharm. 2022;5(4):345–56. https://journal.umpr.ac.id/index.php/bjop/article/view/1411

Bai L, Song P, Su J. Bioactive elements manipulate bone regeneration. Biomater Transl. 2023 Dec 28;4(4):248-269. doi: 10.12336/biomatertransl.2023.04.005. PMID: 38282709; PMCID: PMC10817798. https://pubmed.ncbi.nlm.nih.gov/38282709/

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Published

2025-05-30

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Vol. 14 No. 1 (2025): May

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