The Effectiveness Of Tofu Liquid Waste As A Natural Phytoestrogen For Mandibular Bone Of Ovariectomized Rats

Abstract

Estrogen is a steroid compound that has many benefits for the body's physiology.
Hypoestrogenic women could have periodontitis in their mouth. Hormone
replacement therapy (HRT) is a therapy that used to reduce the disturbance caused
by estrogen deficiency. However, HRT has many side effects. Theoretical study
shows that isoflavones in tofu liquid waste has an element of a steroid compound.
The purpose of this study was to prove the effectiveness of isoflavones in the tofu
liquid waste by examining the microscopic structure, estrogen receptor expression
and MDA levels in rat mandibular bone post-ovariectomy. This research method
use a true experimental laboratory with randomized post test control group design.
Twenty-four female wistar rats were divided randomly into six groups, consisted
of a group of normal rat that does’nt ovariectomized (C1), 4 weeks
ovariectomized rats (C2), 8 weeks ovariectomized rats (C3) but no tofu liquid
waste was given, and 8 weeks ovariectomized rats that given tofu liquid waste at
the end of the 4th week of ovariectomy with three different doses (C4 = 1.2, C5 =
6; C6 = 12 ml / kg). Histopathological slide used to see the changes resulted in a
number of osteoclasts, osteocytes, periodontal ligament width, alveolar bone
height, the amount of estrogen receptor expression and MDA levels. The results
of statistical tests show that there is a difference in the rats between six different
groups (ANOVA, p <0.05) and there is a close relationship between the dose of
the tofu liquid waste with all parameter. The conclusion of this study is tofu
liquid waste could improve the microscopic structure, estrogen receptor
expression and MDA levels in rat mandibular bone post-ovariectomy.