Immunoglobulin-Y Effect On Protein Of Streptococcus Mutans Isolated From Caries And Caries-Free Subjects
DOI:
https://doi.org/10.18196/di.v1i1.514Keywords:
Streptococcus mutans, protein expression of Streptococcus mutans, imunoglobulin-Y anti Streptococcus mutans, caries and free caries subjectsAbstract
The main microbial culprit in dental caries is Streptococcus mutans (S.mutans),virulence of which can be observed by its differential protein expression between
caries and caries-free subjects.The success of Immunoglobulin-Y (IgY) anti
S.mutans as a passive immunization agent in eliminating S.mutans has been
reported. The aim of this study is to analyze the effect of IgY anti S.mutans on the
protein expression of S.mutans isolated from caries and caries-free subjects. Each
dental plaque was collected by swabbing the buccal surface of the first lower
permanent molar of caries and caries-free subjects. The plaques were then
cultured on agar medium TYS20B. After 72 hours, the colonies from each of them
were cultured in liquid medium TYS Broth for 72 hour. Each collected bacteria
(whether from caries or caries-free subjects) were grouped into control and
exposure group. In exposure group, S.mutans was exposed by pre-incubated (for
one hour at 37°C) IgY anti S.mutans for one hour at 37°C. Protein expression of
S.mutans was analyzed with SDS PAGE after the preparation of its antigen and
Bradford protein assay. Our result shows that S.mutans 41.3 kilodalton protein
expression of caries subjects, are up-regulated in comparison to the control group.
Meanwhile, the S.mutans 41.3 kilodalton protein expression of caries-free
subjects, are down-regulated in comparison to the control group. This study
suggests that IgY anti S.mutans up-regulates 41.3 kilodalton protein expression of
S.mutans in the caries subjects. However IgY anti S.mutans down-regulates 4.13
kilodalton protein expression of S.mutans in the caries-free subjects.
Downloads
Published
2012-01-15
Issue
Section
Articles
License
License
Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva (IDJ) is licensed under an Attribution-ShareAlike 4.0 International (CC BY-SA 4.0) license. You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material for any purpose, even commercially. This license is acceptable for Free Cultural Works.
The licensor cannot revoke these freedoms as long as you follow the license terms.
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Copyright
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under an Attribution-ShareAlike 4.0 International (CC BY-SA 4.0) that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
