Identifikasi Kadar Kalsium Pada Serum Tikus Dengan Kelainan Disharmoni Oklusi
DOI:
https://doi.org/10.18196/di.v2i2.581Keywords:
disharmoni oklusi, stress, kadar kalsiumAbstract
Gangguan yang terjadi pada komponen stomatognati seperti berkurangnya kontak oklusal gigi akan menyebabkan terjadinya disharmoni oklusi. Disharmoni oklusi dikenali tubuh sebagai stresor yang mempengaruhi homeostasis dalam tubuh, terutama sekresi hormon dan mineral tulang.
Tujuan. Penelitian ini bertujuan untuk mengetahui kadar kalsium pada serum tikus yang mengalami kelainan disharmoni oklusi.
Metode. Penelitian dilakukan pada 3 ekor tikus putih jantan Sprague dawley untuk masing-masing kelompok. Pada kelompok perlakuan dilakukan pengurangan oklusal di seluruh gigi molar sebesar 2 mm. Sedangkan untuk kelompok kontrol tidak dilakukan apa-apa. Pada hari ke-1, ke-7, ke-14 dan ke-21 dilakukan pengambilan darah melalui vena infraorbita sebanyak 2 cc kemudian disentrifugasi untuk mendapatkan serum. Pengukuran kadar kalsium dengan spektrofotometer.
Hasil. Uji Anova kadar kalsium menunjukkan perbedaan yang signifikan (p<0.05). Kadar kalsium yang paling tinggi terdapat pada kelompok perlakuan hari ke-21.
Kesimpulan. Hasil penelitian menyimpulkan bahwa tikus yang mengalami kelainan disharmoni oklusi berpengaruh terhadap kadar kalsium pada serum.
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