An in Vitro Effectiveness and Evaluation of Sodium Hypochlorite for Orthodontic Buccal Tube Disinfection

Muhamat Muhtar S. Abdurrohman; Dandy Andrea Rainanda; Anggun Feranisa; Ade Ismail Abdul Kodir

doi:10.18196/di.v12i2.16672

An in Vitro Effectiveness and Evaluation of Sodium Hypochlorite for Orthodontic Buccal Tube Disinfection

Muhamat Muhtar S. Abdurrohman, Dandy Andrea Rainanda, Anggun Feranisa, Ade Ismail Abdul Kodir

Abstract

Contamination of fixed orthodontic components caused by non-compliance with hygiene both during the manufacturing and packaging processes can cause cross-infection. Although the literature has demonstrated the need for sterilization or disinfection of fixed orthodontic components prior to insertion in the oral cavity, these are still not widely used in orthodontic practice. This study aims to evaluate bacterial contamination in vitro and the disinfection effect of 0.5% sodium hypochlorite on orthodontic buccal tubes. A total of 12 roth type 1 Molar buccal tubes with slots of 0.020 and still well sealed in the packaging were divided into three groups: group 1 chlorhexidine 2% (n=4), group 2 distilled water (n=4) as control, and group 3 sodium hypochlorite 0.5% (n=4) as treatment. Microbiological and biochemical tests were carried out on the three groups of first molars to detect any bacterial contamination. The buccal tube showed that it was contaminated with bacteria and then disinfected using a 2% chlorhexidine solution, distilled water and 0.5% sodium hypochlorite. Bacterial contamination was detected in all control and treatment groups. The treatment group with 0.5% sodium hypochlorite disinfection showed optimal decontamination (p> 0.05) compared to the negative control group. Therefore, In vitro sodium hypochlorite 0.5% had the effect of reducing bacterial contamination of the buccal tube Roth type 1 molar with 0.020 slots.

Keywords

bacterial contamination; decontamination; sodium hypochlorite 0.5%

Full Text:

PDF

References

Vivek Aithal PR, Akshai Shetty KR, Dinesh MR, Amarnath BC, Prashanth CS, Roopak MD. In vitro evaluation of microbial contamination and the disinfecting efficacy of chlorhexidine on orthodontic brackets. Progress in Orthodontics [Internet]. 2019 May 1;20(1). Available from: http://dx.doi.org/10.1186/s40510-019-0270-4

M H. Assessing Microbial Contamination of As Received Orthodontic Brackets And Wires- An In Vitro Study. International Journal of Advanced Research [Internet]. 2018 Feb 28;6(2):1572–5. Available from: http://dx.doi.org/10.21474/ijar01/6593

Al-Zubaidi HJ, Alhuwaizi AF. Molar Buccal Tubes Front and Back Openings Dimensions and Torsional Play. Journal of Baghdad College of Dentistry [Internet]. 2018 Sep 15;30(3):32–9. Available from: http://dx.doi.org/10.26477/jbcd.v30i3.2529

Bernardo WL de C, Silva JJ da, Höfling JF, Rosa EAR, Boriollo MFG. Dynamics of the seasonal airborne propagation of Staphylococcus aureus in academic dental clinics. Journal of Applied Oral Science [Internet]. 2018 Apr 5;26(0). Available from: http://dx.doi.org/10.1590/1678-7757-2017-0141

Haritha K, Ramesh B, Saralakumari D. Effect of Aloe vera gel on antioxidant enzymes in streptozotocin-induced cataractogenesis in male and female Wistar rats. Journal of Acute Medicine [Internet]. 2014 Mar;4(1):38–44. Available from: http://dx.doi.org/10.1016/j.jacme.2014.01.005

Ramesh N, Katta SS, Kovelakar E, Orthodontist MDSC. To assess the microbial contamination of orthodontic buccal tubes, arch wires and brackets from various manufactures-an invitro study. Published online 2020:34-41.

Suha Saad H, Nidhal GH, Batool Hassan AG. Evaluation of Microbial Contamination of Different Orthodontic as Received Arch Wires from Manufacturers. International Journal of Medical Research &

Health Sciences [Internet]. 2017;6(12):13-18.

Bargale SD, Patel N, Dave BH, Ardeshana A, Mehta DN. Assessment of sterilization technique practices at dental clinics of Vadodara, Gujarat, India. Scholars Journal of Dental Sciences (SJDS) [Internet]. 2016;3(9):240-246.

Deviyanti S. Potensi Antimikroba Photo Activated Disinfection Terhadap Enterococcus faecalis pada Perawatan Saluran Akar Gigi. Cakradonya Dental Journal [Internet]. 2019 Feb 28;11(1):13–22. Available from: http://dx.doi.org/10.24815/cdj.v11i1.13623

Faizi N, Kazmi S. Universal health coverage - There is more to it than meets the eye. Journal of Family Medicine and Primary Care [Internet]. 2017;6(1):169. Available from: http://dx.doi.org/10.4103/jfmpc.jfmpc_13_17

Soares JA, Carvalho MAR de, Soares SMCS, Pereira RD, Brito-Júnior M, Magalhães PP, et al. Pattern of Disinfection of Root Canal Dentin by Alternated Acid-Base Irrigating Regimen. The Scientific World Journal [Internet]. 2019 Jan 1;2019:1–8. Available from: http://dx.doi.org/10.1155/2019/9219238

Kini S, Shetty Sv, Shetty Kh, Kudva A, Kumar P. The efficiency of 2.5% sodium hypochlorite in preventing inoculation of periapical tissues with contaminated patency files: An ex vivo evaluation. Journal of Pharmacy And Bioallied Sciences [Internet]. 2015;7(6):563. Available from: http://dx.doi.org/10.4103/0975-7406.163542

Meshki R, Zarouni F, Sarikhani P. Effect of Sodium Hypochlorite and Sodium Ascorbate on the Shear Bond Strength of Composite to the Dentin of Primary Teeth. Journal of Research in Medical and Dental Science [Internet]. 2019;7(3).

Sinaredi BR, Pradopo S, Wibowo TB. Daya antibakteri obat kumur chlorhexidine, povidone iodine, fluoride suplementasi zinc terhadap, Streptococcus mutans dan Porphyromonas gingivalis (Antibacterial effect of mouth washes containing chlorhexidine, povidone iodine, fluoride plus zinc on Strep. Dental Journal (Majalah Kedokteran Gigi) [Internet]. 2014 Dec 1;47(4):211. Available from: http://dx.doi.org/10.20473/j.djmkg.v47.i4.p211-214

Boal AK, Patsalis FI. Use of Sodium Thiosulfate to Quench Hypochlorite Solutions Prior to Chlorate Analysis. Journal AWWA [Internet]. 2017 Oct;109(10). Available from: http://dx.doi.org/10.5942/jawwa.2017.109.0097

Kuntari LM, Hadriyanto W, Mulyawati E. Perbedaan Daya Antibakteri Klorheksidin 2% dan Berbagai Konsentrasi Sodium Hipoklorit Kombinasi Omeprazole 8,5% Terhadap Enterococcus faecalis. Jurnal Kedokteran Gigi [internet]. 2014;5(2):139-149.

Shah S, Bargale SD, Dave BH, Deshpande AN, Kariya PB, Karri A. Comparison of Antimicrobial Efficacy of (between) 0.2% Chlorhexidine and Herbal Mouthwash on Salivary Streptococcus mutans: A Randomized Controlled Pilot Study. Contemporary Clinical Dentistry [Internet]. 2018; 9(3):440-445

Barros SP, Williams R, Offenbacher S, Morelli T. Gingival crevicular fluid as a source of biomarkers for periodontitis. Periodontology 2000 [Internet]. 2015 Dec 10;70(1):53–64. Available from: http://dx.doi.org/10.1111/prd.12107

Hepyukselen BG, Cesur MG. Comparison of the microbial flora from different orthodontic archwires using a cultivation method and PCR: A prospective study. Orthodontics & Craniofacial Research [Internet]. 2019 Jul 29;22(4):354–60. Available from: http://dx.doi.org/10.1111/ocr.12335

Tenore G, Palaia G, Migliau G, Mohsen A, Rocchetti F, Gaimari G, et al. Evaluation of Photodynamic Therapy Using a Diode Laser 635 nm as an Adjunct to Conventional Chemo-Mechanical Endodontic Procedures against Enterococcus faecalis Biofilm: Ex-Vivo Study. Applied Sciences [Internet]. 2020 Apr 23;10(8):2925. Available from: http://dx.doi.org/10.3390/app10082925

DOI: https://doi.org/10.18196/di.v12i2.16672